The Expression And Significance Of Epithelial-mesenchymal Transition Biomarkers And MiR-134 In Breast Cancer

Purpose:To investigate the prognostic value of epithelial-mesenchymal transition biomarkers and miR-134 in breast cancer and their clinical significance.Methods:1.A systematic search of PubMed, the ISI Web of Science, Cochrane Library, China BioMedicine Literature (CBM) and China National Knowledge Infrastructure (CNKI) was performed with the last report updated on November 14,2014 to extract data and identify the relation between the immunohistochemical expression of E-cadherin, vimentin and clinicalpathological features as well as outcome of non-lobular breastcancer.2.The expression of E-cadherin, N-cadherin, vimentin in 97 breast cancer tissue samples was detected by MaxVision two-step method of immunohistochemistry and the expression of miR-134 was detected by in situ hybridization, to analyse the association between their expression, and the relationship between their expression and clinicopathological characteristics.Result:1.A total of 25 studies comprising 7,217 non-lobular breast cancer patients were included. The overall analysis showed that both down-regulation of E-cadherin and up-regulation of vimentin had unfavorable impacts on overall survival (OS) (RR=2.12, 95%CI:1.61-2.78, P<0.00001 for E-cadherin and RR=1.35,95%CI:1,16-1.57, P=0.0001 for vimentin) and disease-free survival (DFS) (RR=1.77,95%CI:1.48-2.12, P<0.00001 for E-cadherin and RR=1.44,95%CI:1.21-1.71, P<0.0001 for vimentin). Both E-cadherin low-expression and vimentin over-expression were significantly correlated with higher histological grade, ER negativity and PR negativity. Meanwhile, down-regulation of E-cadherin was also associated with larger tumor size and higher TNM stage, and up-regulation of vimentin was significantly correlated with HER-2 positivity.2.Both low-expression of E-cadherin and up-expression of N-cadherin and vimentin in 97 breast cancer tissue samples were positively correlated with higher histological grade, lymph node involvement and HER-2 overexpression, while negatively associated with the expression of ER and PR. The expression of miR-134 was negatively associated with higher histological grade, HER-2 overexpression, low expression of E-cadherin as well as high expression of N-cadherin and vimentin.Conclusion:1.The systematic review and meta-analysis indicated that EMT biomarkers act as predictive factors of malignant progression and poor survival in non-lobular breast cancer.2. The expression of EMT biomekers in breast cancer tissue was significantly associated with the progression of neoeplasm. Meanwhile, the expression of miR-134 negatively related to both the malignant progression and EMT, indicated that it might be a potential bio-marker for breast cancer.