| Objective:Colorectal cancer is one of the high incidence of the most common tumor in the world, the incidence of colorectal cancer in recent years, showing a rising trend.According to the global cancer statistics report: colorectal cancer in women accounted for the second common types of cancer, ranked third in the male common types of cancer. Colorectal cancer is by the interaction between multiple factors and multiple genes and gradually developed as a result, its precise mechanism has not been fully elucidated.BMI-1(B-cell-specific moloney murine leukemia virus insertion site 1) gene belongs to multiple comb(polycomb group genes, Pc G), an important member of the family, a large number of literature, a variety of tumor occurrence and development process are associated with BMI-1 gene expression, and is associated with the prognosis of tumor, and argues that it is a "cancer stem cells" in the tumours. We found that BMI-1 was overexpression in colorectal carcinoma, and had a relationship with tumorigenesis of colorectal carcinoma.In the work, we examined the expression of BMI-1 in tissues by q RT-PCR(real-time quantitative reverse transcription-PCR, q RT-PCR), provide a theoretical foundation for understanding the role of BMI-1 take part in the pathogenesis of colorectal carcinoma.Methods:1 There are 41 cases fresh specimens were collected, included carcinoma tissues and normal mucous tissues. All the specimens were come from the First Hospital of Hebei Medical University and Fourth Hospital of Hebei Medical University between September, 2002 and September 2012. All the patients were not received chemotherapy and radiotherapy before operation.2 The expression of BMI-1 in 41 cases cancer tissues and correspond normal tissues were detected by q RT-PCR, and analyzed the relationship between the expression of BMI-1 and clinicopathologic feature.3 Flow cytometry was performed in control group and experimental group to compare the difference.4 All the results were analyzed by SPSS19.0 and Graph Pad Prism 5.0, the results were showed by median(quartile), Wilcoxon test was used to analyzed paired samples, One-way ANOVA was used to analyzed multiple groups comparison, Two-way ANOVA was used to analyzed repeated measure data. Kaplan-Meier survival curve was drawn. Survival time was between the operation day and the last follow up, calculated on month. Multi-factor survival analysis was performed by Cox regression analysis. P<0.05 was regarded as statistical significance.Results:1 The expression of BMI-1 in colorectal cancer tissues and normal tissues were 0.0082(0.0047,0.0241) and 0.0024(0.0009,0.0070), respectively. The expression of BMI-1 in cancer tissues was higher than in normal tissues(P<0.0001), the difference was statistically significance.2 The expression of BMI-1 in large tumor group(equal or greater than 5cm) were higher than small group(less than 5cm), the difference was statistically significance(P=0.0236). BMI-1 was independent with lymph nodes metastasis, age, gender, depth of invasion.3 Survival rate analysis shown that the survival rate of patients was correlated with lymph nodes metastasis(P=0.0002)and depth of invasion(P=0.0137), the difference was statistically significance.4 Cox multiple factor survival analysis shown that the lymph nodes metastasis was the independent risk factor that impacted the survival rate of colorectal carcinoma patients(P=0.003). The gender, age, tumor volume, BMI-1 level and depth of invasion were no relevance with patient survival rate.1 The expression of BMI-1 is high level in colorectal carcinoma, suggesting that BMI-1 may probably have a potential oncogene activity.2 The expression of BMI-1 is up-regulated even more in the tumor which is equal or greater than 5cm, suggesting that it has significance in judging grade malignancy and prognosis of colorectal carcinoma. Conclusions:... |
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