Malaria In Pregnancy Affect On Growth And Cellular Immune Responses Of Generation Mice

Purpose: To establish an animal model of pregnant mice with malaria, to observe the influence of pregnant mice infected with the malaria parasite on the growth and cellular immune responses of the filial mice, to study the influence of pregnant filial mice contacting plasmodium falciparum on their susceptibility, so as to deepen our knowledge and understanding of the interaction between parasites and the hosts as well as the pathogenic mechanism of pregnancy malaria.Methods:(1)Choose and put clean adult Kunming mice in one cage, mating them and ensuring the pregnancy. Give the pregnant mice intraperitoneal inoculation of six different doses P.berghei respectively on the 7th, 14 th and 16 th day and observe all the cases of abortion of these pregnant mice.(2)On the fourteenth day of pregnancy, give the pregnant mice intraperitoneal inoculation of P.berghei 1×106 as the experimental group, inoculate 0.9%Nacl of the volume same as the control group, feeding the pregnant mice to spontaneous delivery. At the time of delivery, observe physiological indicators such as birth weight and litter size of the neonatal mice and take the tail blood to make blood smear. Observe the CD4/CD8 of t-cell subset changes in their thymuses and spleens on the 1st, 3rd, 5th days after their birth.(3)When the two groups of newborn mice grow to the 4th week, observe physiological indicators of the P.b exposure and the change in CD4/CD8 T cells in the thymuses and spleens of the fetal period mice.(4)Give P.b 1×106 to the two groups of filial mice and observe their red blood cell infection rate and survival time for fourteen days after the vaccination. On the third day after vaccination, observe at random the two groups’ spleen cell proliferating reaction to P.b antigen stimulation.Results:(1)The cases of fetal rats abortion of pregnant rats on the 14 th day infection group are significantly fewer than those of the 7th day group(P<0.05) but significantly more than those of the 16 th day group(P<0.05). In the group of the 14 th day, 1×106 aborting pregnant mice of are much fewer than 8×106 mice(P<0.05), but have no statistical significance compared with the other group(P>0.05).(2)The result of the experimental group of newborn mice blood smear is negative, but its birth weight andsurvival number decrease significantly compared with the control group(P<0.05). On the 1st, 3rd, 5th days after their birth, comparing the proportion of CD3+CD4+CD8-T cells in thymus and spleen, the experimental group filial mice significantly increases(P<0.05). There is no statistical significance compared at various points in the spleen.(3)The weight of filial mice of with P.b exposure of four weeks is much lighter than the ones with no exposure(P<0.05). The proportion of CD3+CD4+CD8-T cells in the thymuses and spleens increases significantly(P<0.05) while the the proportion of CD3+CD4-CD8+T cells decreases significantly(P<0.05).(4)Filial mice with P.b exposure 4 weeks, given intraperitoneal inoculation of P.b 1×106, after observation of 14 days, the survival time of the exposure group is significantly shorter than that of the non-exposure group(P<0.05)and the red blood cell infection rate also decreases significantly(P<0.01).On the third day of vaccination, comparing the two groups on the the proportion of CD3+CD4+CD8-T cells in their thymuses and spleens, that of the exposure group increases significantly(P<0.01), while the proportion of CD3+CD4-CD8+T cells is significantly reduced(P <0.05).Conclusion:(1)It is helpful to establish animal models with pregnant malaria mice when giving the mother mice peritoneal inoculation on the 14 th day of pregnancy.(2)Mother mice in pregnancy infected with P.b may influence the growth and the proportion of CD4/CD8 T cells subgroup in thymuses and spleens of the filial mice.(3)Mother mice in pregnancy infected with P.b have changed the filial mice’s survival time and terminal erythrocyte infection rate.