Effect And Mechanism Of 4-phenylbutyric Acid On Vascular Permeability Of Pulmonary In Sepsis Rat

Objective:To approsch the effect and mechanism of 4-phenylbutyric acid on vascular permeability of pulmonary in sepsis rat.Methods:With cecal ligation and puncture(CLP) imitated sepsis model,we observed the variability of the pulmonary vascular permability,endoplasmic reticulum stress marker ptroteins such as GRP78,GRP94 and CHOP of sepsis rat model after applied4-phenylbutyric. With culturing pulmonary vein endothelial cells,we observed the effect of F-actin after giving LPS or 4-PBA.Results : In comparison with the sham group,sepsis raised the pulmonary vascularpermability and the expression of the endoplasmic reticulum stress marker ptroteins,but 4-phenylbutyric acid was exhibit the opposed effect. In comparison with the sham group, Lipopolysaccharide increased the expression of F-actin which induced cell constraction. 4-phenylbutyric acid was amendmented this phenomenon.Conclusion:1. Sepsis can stimulate endoplasmic reticulum stress in vascular endothelial cells. ERS to attend the process of vascular permeability increasing. Protein GRP78、GRP94 and CHOP are the important molecules in the process of vascular permeability increasing after Sepsis.2. Endoplasmic reticulum stress may adjust the vascular permeability via regulation of the formation of stress fiber.3. 4-PBA may restrain the formation of stress fiber and depress the vascular permeability via inhibiting endoplasmic reticulum stress.