| Objective: In this study, rats were mainly from the increase and reduce physical fracture fracture COX-2 content from short-term and long-term use of early drug-related fracture healing, for clinical fractures in patients with conservative and surgical treatment to accelerate fracture healing and reduce the rate of nonunion provide new treatment programs.Methods: 1 choose 48 male rats using the rat femoral transverse intramedullary nail fixation model and randomly divided into three groups of fracture. The A group was the blank group(16 rats); group B for COX-2 inhibited group(16 rats); group C was the experimental group(increasing the content of COX-2 rats 16 only). Establishment of experimental model of fracture of domestic rats mostly adopts simple closed bone method, postoperative is not fixed or simple external fixation, but closed bone fracture type is difficult to determine the unified, contrast and poor. This experiment adopts open made fracture type. Although larger wounds, but the experimental animal of each group the same degree of trauma, reliable fixation. Keep the fracture healing were compared, the results of the experiment will have more significance2 fractures after modeling to A group were given saline intravenously; group B with celecoxib(COX-2 inhibitor) suspension 24 mg/kg- D. Group C was given(COX-2 reagent 1u M/kg- D) intravenous administration. In every group,fracture after modeling radiological examination 2, 4, 6, 8 weeks, callus growth evaluation, and the fracture healing was blinded scoring. Every 2weeks in each group were sacrificed 4 mouse tissue examination, HE staining and image analysis system measured application end density of trabecular bone fracture. At the same time rats in each time segment extraction 1ml serum BMP-2 examination. Using SPSS19.0 statistical software for statistical analysis.Results: 5 rats due to anesthesia, postoperative wound infection and internal fixation failure and other reasons removed and re-additions.1. radioactive check: A group was the blank group, the growth of callus, the fracture line was clear in 2 weeks, 4 weeks, 6 weeks of fracture lines blurred, fracture line began to disappear and the emergence of callus plasticity, fracture healing 8 weeks basic. B group was COX-2 inhibited group callus morphological growth later, 4 weeks after fracture bone broken line blurred shadow is not clear, callus growth was less, and 6weeks after fracture line is not very clear, 8 Zhou Yuhe good and fracture callus absorption plastic etc.. COX-2 inhibited group short-term(2.4week) and long-term(6.8 weeks) treatment of fracture healing difference compared with the blank group significantly(P<0.05). Group C was the experimental group 2 weeks of fracture line slightly fuzzy, 4 weeks and the emergence of a small amount of callus and the fracture line is not obvious, 6 weeks, callus growth model and plastic. In 8 weeks the healing of fractures has been close to normal structure. The experimental group of short-term and long-term medication than A group obviously(P<0.05) in fracture healing.2. Histological examination: A group was the blank group after operation of fracture end around the bone trabecula were arranged in order, callus morphology to fibrous tissue and a small amount of cartilage tissue, B group for the inhibition of COX-2 group 4 weeks(short-term medication)in trabecular bone is relatively thin, see growth more cartilage cells, bone callus morphological see lots of fiber cartilage the organization is lower than that of control group(P<O.05), group C was the experimental group short-term medication trabecular bone cartilage cells closely, vigorous growth, callus morphology of cartilage tissue than in fibrous tissue compared with the control group was significant difference(P<0.05), B group of 8 weeks(long-term use) bone little Liang Xishu, arranged in disorder, trabecular bone discontinuity more, hypertelorism, callus morphology is mainly expressed in tissues of cartilage tissue and different amount of mature bone was significantly lower than the control group(P<O.005); group C(8 weeks) long-term medication group higher bone density, bone trabeculae arranged in neat rows, callus morphology show not mature bone tissue with mature bone tissue and was significantly higher than the group A(P<0.05).3.bone morphogenetic protein(BMP-2) concentration measurement of all groups after operation: BMP-2 assay, B, C group of short-term and long-term treatment group compared with the control group with significant difference(P<0.05).Conclusions: 1 early(4 weeks) and long-term(8 weeks) using COX-2 inhibitors,significantly reduce the fracture healing, fracture callus growth and prolong the time of fracture healing and increased nonunion rates,increased bone nonunion;2. Increase in vivo COX-2 may promote healing fracture healing is more conducive to long-term use, reduce the rate of nonunion.;... |
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