Effects Of LncRNA-LET Gene Transfection On Proliferation,apoptosis,invasionandmetastasis Of Esophageal Squamous Cell Carcinoma

Objective: To investigate the effects of lnc RNA-LET gene transfection on proliferation, apoptosis, invasion and metastasis of esophageal squamous cell carcinoma Eca109 and TE-1 cell lines.Methods: The expression of lnc RNA-LET m RNA in primary ESCC and the matched normal tissues(48 cases) was determined by quantitative RT-PCR. The relationship between tumor tissue lnc RNA-LET expression and pathological characteristics in clinic will be analyzed. Lentivirus infection technique was employed to up-regulate the expression of lnc RNA-LET in Eca109 and TE-1 cell lines. The cell proliferation and viability was measured by MTT assay. The effect of lnc RNA-LET on cell apoptosis was evaluated by flow-cytometric analysis. Transwell and wounding healing assay were used to detect the cellular invasion and metastasis of esophageal squamous cell carcinomas.Results: Expression of lnc RNA-LET was decreased in primary ESCC tissues compared to paired normal tissues. Analysis indicated that the expression level of lnc RNA-LET was positive correlated with pathological grade and negative correlated with the TNM stage and lymph node metastasis. Over-expression of lnc RNA-LET was validated to inhibit the cell proliferation, metastasis and invasion of ESCC cells, and modulate p53 expression in human ESCC cell lines in vitro.Conclusions:These results revealed that lncRNA-LET may play an important role in the regulation of tumor progression and metastasis and serve as a tumor suppressor in human ESCC with therapeutic potential for the treatment of human ESCC.