To Research The Effects Of Sodium Houttuyfonate With Bufei Decoction On Immunologic Function In Lung Deficiency Rats Induced By Pseudomonas Aeruginosa Biofilms

Objective: To research the effects of Sodium Houttuyfonate(SH) with Bufei decoction(BFT) on immunologic function in lung deficiency rats induced by Pseudomonas aeruginosa biofilms, provides the basis of Two drug combination therapy infection caused by lung deficiency syndrome.Methods: The chronic respiratory infection, or the model of lung-qi deficiency syndrome, was induced by PA with nasal drip, the stimulations of cold and fatigue in rats. Observed the effects of symptoms of rats after drug intervention. Using Blood rheometer and blood gas analyzer to detect hemorheology, blood gas analysis,respectively. Observed the symptoms and pathological changes in lung tissue by microscope, and rat body weight, organ index of changes in rats of each groups.Detected IL-1β、IL-6、IL-10、β-D2、TNF-α、IFN-γ levels in bronchoalveolar lavage fluid with Enzyme-linked immunosorbent assay; RT-PCR and Western-blotting were used to detect the expression quantity of My D88, TLR4, NF- κB, p- NF- κB, IκB, p- IκB, Gas6,Pros changes of protein in rat lung tissue. MTT methods was used to detect pseudomonas aeruginosa biofilm and drug effects on the proliferation of A549 cells in vitro. Apoptosis protein of Bcl-2 and Bax expression were detected by Western-blotting.Results: qi deficiency of chronic bronchitis pulmonary models were successfully constructed, induced by pseudomonas aeruginosa biofilms with cold fatigue stimulating. The rat lung qi deficiency symptoms obviously. Compared with control group, the lung tissue pathology test was chronic inflammation. Model group rats weight increase slowly, viscera index decreased, Rat whole blood viscosity(high shear, low shear), plasma viscosity, erythrocyte aggregation index, erythrocyte hematocrit and fibrinogen were significantly higher( P<0.01), Blood p H, Pa O2,Sa O2 % values are different degrees of reduction, Pa CO2 a significant rise in value,statistically significant difference(P < 0.01). The IL-1β、IL-6、TNF-α、IL-10、IFN-γ、β-D2 in bronchoalveolar lavage fluid and My D88/TLR4-NF-κB were high expression.compared with normal control group, Gas6 / Pro S- TAM system significantly low expression(P<0.05).The treatment group rats lung qi deficiency symptoms gradually disappear with two medicines joint intervention. There was no severe inflammatory reaction in the lung tissue.Compared with model group, the rats weights and Viscera index increased significantly, Combination groups have significantly improvement in the blood rheology and gas indexes, and better than the single drug groups(P<0.01). The lung tissue pathology test was chronic inflammation.The IL-1β、IL-6、TNF-α、IL-10、IFN-γ 、 β-D2 of bronchoalveolar lavage fluid and My D88/TLR4-NF-κB were high expression. compared without treatment group, Gas6 / Pro S- TAM system significantly low expression(P<0.05).Sodium houttuyfonate combinated with Bufei decoction(SH&BFT) could improve the expression of Bax protein in vitro. Precipitated the Bcl-2 / Bax was reduced, induced the apoptosis of A549 cells to inhibit pseudomonas aeruginosa biofilm.Conclusion: Qi deficiency of chronic bronchitis pulmonary models were successfully constructed, induced by pseudomonas aeruginosa biofilms with cold fatigue stimulating. The rat organs indexs, blood rheology and blood gas were Significant improvement after treatment with Sodium Houttuyfonate with Bufei decoction. Two drugs combined could significantly inhibit TLR4-NF-κB signaling pathways of inflammatory factors inflammation of the lung qi deficiency syndrome, Promote the negative regulation Gas6 / Pro S- TAM system, the expression of intervention for the treatment of chronic bronchitis, pulmonary qi deficiency syndrome. It play an active role in treatment of qi deficiency of syndrome chronic bronchitis.