Correlation Between The Expression Of OGR1 In Peripheral Blood Cells And Hypoxic Pulmonary Hypertension

Aims:To detect the expression changes of proton-sensing receptor in human peripheral blood cells in hypoxic pulmonary hypertension patients and healthy people, and analyze the correlation between this change and clinical pathological, discuss the role of the proton-sensing receptor in hypoxic pulmonary hypertension. This study provides the theoretical foundation about function of the proton-sensing receptor in pulmonary hypertension, and provides new research ideas for clinical pulmonary hypertension prevention and control. Methods:Collection of 30 cases of pulmonary hypertension patients and 30 cases of clinical general physical examination, including gender, age, blood gas analysis, pulmonary function, cardiac color ultrasound; while collecting peripheral blood samples, extracting total RNA, using real-time fluorescent quantitative PCR to detect peripheral blood G2A, GPR4, TDAG8 and OGR1 mRNA expression level. Enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) to detect serum tumor necrosis factor-α (tumor necrosis factor-α, TNF-α) and interleukin-6 (interleukin-6, IL-6) levels. Results: ① The two groups were not significantly differential in gender and age (P>0.05)。② Blood gas analysis results suggest:on average, PaO2 52.13+6.33 mmHg, PaCO2 54.54±20.88 mmHg in Hypoxic pulmonary hypertension group; PaO2 77.82±3.95 mmHg, PaCO2 39.40±3.39 mmHg in health controls.PaO2、PaCO2 comparative differences between the two groups have statistical significance, (P< 0.01). ③ Lung function result:FEV1% was 39.60+12.86, FEV1/FVC average was 55.59±7.76 in hypoxic pulmonary hypertension group;health control group FEV1% was 109.00+18.30, FEV1/FVC average was 82.60 ±3.66. FEV1%, FEV1/FVC comparative differences between the two groups have statistical significance, (P< 0.01). ④ Hypoxic pulmonary hypertension group pulmonary artery systolic pressure average was 60.03±15.79 mmHg, healthy control group pulmonary artery systolic pressure average was 24.63±3.23 mmHg, The differences between the two groups were statistically significant, (P< 0.01).⑤ The expression of TDAG8 GPR4、G2A of peripheral blood in patients with pulmonary arterial hypertension has increased dramatically than normal(P<0.01). ⑥　Hypoxic pulmonary hypertension group TNF-a average content of 0.26+0.09 ng/L, IL-6 average content of 43.26±21.07 ng/L, has a significant difference compared with the healthy group, P<0.05.Conclusion: ① tDAG8, GPR4, G2A mRNA is highly expressed in patients with pulmonary hypertension, and it suggests that proton-sensing receptors are involved in the perception of hypoxic pulmonary hypertension. So combining detection of three levels is the molecular markers to test pulmonary hypertension.② Pulmonary artery pressure、the level of TNF-α IL-6 and the expression of some proton sense receptors have relevance in the hypoxic pulmonary hypertension group.This further provides theory basis for molecular pathways for the treatment of PAH.