Study Of Locomotive Behavior Responses In Individual Caenorhabditis Elegans Based On Microfluidic Droplet Injection

Droplet microfluidic technology had been used for single cell high-throughput drug screening, however, manipulations of droplets for single C.elegans high-throughput drug screening were still by passive manners(passive methods do not rely on peripheral power sources or components to perform droplets manipulations. Instead, they depend on the channel design to control the droplets operations), advantages unique to the droplet-based system such as its throughput and automation had not been highlighted. For the purpose of automatic and flexible manipulation of the C.elegans droplet, the self-assembly droplet high-throughput detection system was used to achieve the droplet injection by active manner(In contrast to passive fission methods, active manners may rely on external power) which constituted the droplet high-throughput drug screening system at the C.elegans level. The main contents were as follows:At first, single worm encapsulation was accomplished for the advanced research.For the C.elegans droplet manipulation automatically, the microfludic droplet injection system was designed to operate the droplet fusion by active manners, the results shown that the microfluidic injection system made the droplet injection more manageable、automatic and accurate.Finally, on the basis of above work, the two integrated system was developed to study the characteristics of Cu2+ induced neurotoxicity to C. elegans. The results shown that the C. elegans locomotive behavior defects caused by the copper exposure had a concentration-dependent manner compared to the tradition multiwell experiment.