| Objective:To investigate the relationship between Graves’disease(GD) and protein tyrosine phosphatase non-receptor type 22 (PTPN22) C1858T gene polymorphism in Han nationality from Anhui of China. Furthermore,Compared with prior research, explore if there is racial difference of PTPN22 C1858T.Methods:82 GD patients was collected from Anhui province han nationality in our visitors, (male, n=34, female, n=48), whose average age is 33.48±8.44; 70 health individuals whose average age is 34.82±6.73 were enrolled into the study as control group, matched for age and sex (male, n=31, female, n=39).Collected the blood samples, The PTPN22 gene C1858T alleles were detected by Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) technology. comparisons of genotype and allele frequencies were made between GD patients and normal group.Results:All of the patients and controls were CC genotype, the T allele was not detected.Conclusion:1. PTPN22 C1858T gene polymorphism does not play a crucial role on Graves’disease in Anhui population.2. There are differences in different races and regions for PTPN22 C1858T distribution,the han people in Anhui have not found the existence of the 1858 T allele.Object:To investigate the impaction of methimazole and 131-1 therapy on Peripheral blood lymphocyte early apoptosis in patients with Graves’ Disease. To compare the differences between early apoptosis in PBL of patients with two different treatments.Methods:We chose a total of 74 GD patients. They were subdivided into three groups:29 subjects were newly diagnosed or untreated nearly a year in patients with recurrent Graves’ disease(A group)(male, n=7; female, n=22)whose average age is 38±14.36; 20 subjects were in euthyroidism within the next six months of 131-Ⅰ therapy and had not taken any anti-thyroid drugs(B group) (male, n=5; female, n=15) whose average age is 36±10.20; 25 subjects were in euthyroidism during methimazole treatment with Maintenance dose (C group)(male, n=8; female, n=17) whose average age is 36±12.34. In addition,23 euthyroidism health individuals matched for age and sex were allocated to control group (D group), (male, n=8; female, n=15) whose average age is 35±16.30. The blood samples were collected for measuring thyroid function and the related antibodies.Apoptotic cells were detected using the Anexin-V/PI kit by flow cytometry.Results:1.The percentage of early apoptotic in PBL of group A (8.17±2.92)、group B (12.92±3.99%)and group C (18.19±3.52%)were all significantly higher than that of group D (2.67±1.36%) (F= 54.238, P< 0.05);2.The early apoptosis in PBL of group B (12.92±3.99%) and group C (18.19±3.52%) were both significantly higher than that of group A(8.17±2.92%) (P < 0.05);3. The early apoptosis in PBL of group C (18.19±3.52%) was higher than group B (12.92±3.99%) as well((P< 0.05)).4. Early apoptosis in PBL of A group was positively correlated with with FT3, FT4 (r=0.483,r=0.361,P<0.05) and negatively correlated with TSH (r=-0.512, P<0.05)Conclusions:1.patients of Graves’disease had a significantly increased early apoptosis of PBL. Early apoptosis of PBL and thyroid hormone levels were positively correlated, suggest that thyroid hormones have the potential to induce apoptosis of peripheral blood lymphocyte.2. There was a increased early apoptosis of PBL in patients with methimazole and 131-1 therapy which suggest that methimazole and 131-1 may both induce apoptosis in PBL and improve the Autoimmune condition.3. compared with 131-1 therapy, treatment by methimazole makes a greater influence on peripheral blood lymphocyte early apoptosis. |
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