Expression Of Prostaglandin Transporter In Colorectal Cancer Tissues And Its Relationship With Clinicopathological Features

Objective To investigate the expression of prostaglandin transporter(PGT) in colorectal cancer(CRC) tissues and its relationship with clinicopathological features.Methods The m RNA and protein levels of PGT were determined by real-time PCR、Western blot and immunohistochemical methods in 80 patients with colorectal cancer and adjacent normal tissue,and its relationship with clinicopathological features was analyzed.Results Compared with the adjacent normal tissue of colorectal cancer,The m RNA relative expression of PGT in colorectal carcinomas was 0.57±0.33 vs. 2.33±1.20 P<0.01,the PGT/ glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gray value of protein expression in colorectal carcinomas was 0.45±0.16 vs. 0.78±0.23 P<0.01,and the integral optical density(IOD) results of immunohistochemisty was 718.7±359.4 vs. 10412.0±6423.3 P<0.01,the average optical density(AOD) results of immunohistochemisty was 0.03±0.01 vs. 0.12±0.09 P<0.01.They were significantly lower expression in colorectal carcinomas.The m RNA and protein low expressions of PGT in patients with colorectal cancer were associated with depth of invasion(T3-T4) and tumor Node Metastasis(TNM) stage(stage Ⅲ-Ⅳ)(P<0.01),but not associated with gender、age、tumor location and differentiation degree(P>0.05).Conclusion The expression levels of PGT m RNA and protein in the colorectal cancer tissues were significantly low. It was associated with depth of invasion and late stages.