Expressions Of PIN1 And NANOG In Human Gliomas And Their Significance

Objective To study the subcellular location of PIN 1 and NANOG in glioma cells and their expressions in glioma tissues, and explore their significance in the pathogenesis of gliomas, and influence on glioma biological activities, for further study on the subsequent PIN1- NANOG related pathways of epigenetics intervention to lay the foundation. Methods ①Immunohistochemical method to detect 84 cases of gliomas and 10 cases of normal brain tissue specimens of PIN1, NANOG expression. ②Mu at 1.0 mol/L Pi B processing U87 cells for 24 h 1.0 mu mol/L Pi B group, U87 cells as a control group without any processing immunofluorescence double dye detection the expression of cell PIN1, NANOG, rt-pcr and Western blotting test cell PIN1, NANOG m RNA and protein expression. ③By determined by MTT, Transwell cell migration methods of experiment, nude mice into tumor detection PIN1- NANOG related pathways expression suppression effects on glioma cell body inside and outside the biological activities. Results ①The positive protein expression rate of PIN1 and NANOG in gliomas with different grades was significantly different, the higher the malignancy grade, the higher the rate of positive protein expression of PIN1 and NANOG(P<0.05); ②Compared with the control group, m RNA and protein expression of Pin1 and NANOG gene were significantly down-regulated in the experimental group cells; ③ immunofluorescence staining results show that the NANOG+, PIN1133+, NANOG+/PIN1+cells was significantly reduced; ④ Determined by MTT results showed that dosing group cell inhibition rate obviously, and with the dru Transwell test cell migration and invasion ability decreased obviously.g dose and action time correlation, ⑤Transwell test cell migration and invasion ability decreased obviously. Pi B treatment group U87 cells in nude mice subcutaneously into tumor ability also significantly lower(P < 0.05) Conclusion: ①PIN1 and NANOG play important roles in the pathogenesis of human gliomas and are related to the malignancy; ②At the same time we found that PIN1 may participate in the regulation of NANOG, there is a pathway between the two genes; ③the glioma cell proliferation ability is suppressed by down-regulated PIN1-NANOG expression.