| OBJECTIVE:Survey of guangxi region beta thalassaemia major pa-tients with thyroid function normally, the heavy beta thalassaemia patients iron overload and the relationship between thyroid function, to test whether the body iron overload level have a certain influence on the thyroid function, to provide basis for clinical treatment of Mediterranean anemia and endocrine related com-plications.METHODS:By focusing on clinical collection, collection, in May 2009 to May 2014, China’s guangxi zhuang autonomous region (including nan-ning, baise, guilin, yulin, and a small number of guangxi region) genotype in-specting diagnosed with heavy beta (equivalent to "beta"), a total of 300 patients with poor into the observation object (among which 197 were male, female 103 cases,2-25), the concentration of selected 85 cases of healthy control group (53 cases of male, female 62 cases,3-15 years of age), the comparison and analysis between the 2 groups of growth and development situation, the level of thyroid function (FT3, FT4, TSH), and the poor group of iron overload condition and its relationship with thyroid function.By intraperitoneal injection of dextran iron iron overload Mongolian gerbils laboratory model is set up 18,18 and set up the control group, control group intraperitoneal injection of equal dose of normal saline, kept the same condition the 14 weeks (14 w),16 weeks (16 w), after 18 weeks (18 w), according to each batch of 6 in each group, partial thyroid tissue, formalin fixed, paraffin embedding, sectioning, respectively, for HE staining, the Prussian blue iron stain, TSH resistance -r immunohistochemical staining, com-pare and contrast the experimental group and control group of Mongolian ger-bils dyeing results.RESULTS:1) 300 cases of patients with heavy beta to a poverty exists low height 111, accounting for 37.5%;Control group,85 cases of objects have 5 height is low, accounting for 5.9%.To the poor group in 65 patients (22.0%) appeared low weight, the control group had 4 cases (4.7%) of low weight.2) in 300 cases of thyroid function in patients with poor measurement (TSH, FT4, FT3) 26 patients (8.67%) of "subclinical hypothyroidism" type, there are 2 cases (0.66%), characterized by "hypothyroidism", to the TSH level of lean group than in control group (P= 0.001);The control group did not appear subclinical type or clinical hypothyroidism.3) 208 patients with heavy beta to lean in a state of severe iron overload, accounted for 70.27%, SF, CMR is found no significant correlation between thyroid function.4) iron overload Mongolian gerbils thyroid Prussian blue iron stain with the control results are negative;Iron overload gerbils thyroid tissue immunohistochemical (TSH resistance-R) Mean Density value was 0.079 ± 0.166, the control group gerbils Mean Density value was 0.029 ± 0.007, the scheme of iron overload group Density value is greater than the control group significantly (P< 0.001).CONCLUSIONS:1) in guangxi region heavy beta to lean the inci-dence of subclinical hypothyroidism patients (8.67%) is significantly higher than control group in the crowd.For heavy beta thyroid function in patients with poor suggested that early monitoring, early detection, early intervention, early treat-ment.2) teenagers to poor patients with short stature, low body weight are more common, higher than the general population of the same age and sex, need reg-ular monitoring and early clinical intervention.3) iron overload gerbils is signif-icantly higher than that of control group, serum ferritin values iron overload gerbils thyroid tissue found no significant iron deposit;Iron overload gerbils thyroid gland bubble and basal organization immunohistochemical TSH -r ex-pression positive rate was significantly higher than control group in gerbils. |
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