| Objective: Psoriasis is a common chronic inflammatory skin disease characterized by abnormal differentiation and excessive proliferation of keratinocyte(KC).At present,its exact causes are not fully known.But factors like heredity,environment and immunity may involved in the occurrence and development of psoriasis. It indicated that pro-inflammatory such as interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) play an important role in the occurrence and development of process. IL-37 is considered as a novel anti-inflammatory cytokine which involved in the immune and inflammatory response by inhibiting pro-inflammatory cytokines and chemokines, regulating gene transcription.At present,IL-37 plays an important role in a variety of inflammatory and immune related diseases.but, there was no reports about the study of IL- 37 on human psoriasis.The research focuses on how Recombinant Human IL-37(Hu IL-37) influence cell proliferation and the expression of IL-6,TNF-α,IFN-γ mRNA in KC in the lesions of psoriasis vulgaris, and preliminarily explores the effects of Hu IL-37 on its anti-inflammatory and anti-proliferation in psoriasis, which aims at providing a new way for psoriasis treatment. Methods: 1. collected 5 patients with psoriasis vulgaris in active stage,then cut some skin in their lesion areas and cultured it to get keratinocytes.2.The KC was divided into four groups,named A,B,C and D. Added an increasing concentration of Hu IL-37 interventing cells into three groups, Group A with 10ng/ml, Group B with 100ng/ml, Group C with 200 ng/ml. Group D(control group) was for no intervention.Then collected the cells in each group in 24 hours,to detect KC proliferation in each group by CCK-8;to detect the expression of IL-6, TNF-α and IFN-γ m RNA in cells among each group by fluorescence quantitative PCR. Results: 1.KC can be isolated and cultivated with two-step digestion and Serum-free culture method. 2. The influence of Hu IL-37 on KC proliferation in the lesions of psoriasis vulgaris: with 24-hour’s intervention by different concentrations of Hu IL-37, the absorbance of KC in Group B and C were less than that of Group D(P<0.05), especially in the Group C; the Group A compared with Group D was no significant statistical difference(P>0.05).the inhibition on KC proliferation of Group A,Group B and Group C were(10.49 ±5.65) %,(25.47 ±6.13) % and(42.49 ± 3.81) %, which Group C are higher than Group A and Group B(P<0.05), and Group B was higher than Group A(P<0.05). 3.The influence of Hu IL-37 on the expression of IL-6,TNF-α, IFN-γ m RNA in KC in psoriasis vulgaris lesions: with 24-hour’s intervention by different concentrations of Hu IL-37,the IL-6 m RNA in Group A, B and C were less than that in Group D(P<0.05), and Group C was less than Group A and Group B(P<0.05),Group B was less than Group A(P<0.05). the TNF-α m RNA in Group B and Group C were less than that in Group D(P<0.05), the TNF-α m RNA in Group A compared with Group D was no significantstatistical difference(P>0.05),the TNF-α m RNA in Group B was less than that in Group A(P<0.05). the IFN-γ m RNA in Group A, B and C were less than that in Group D(P<0.05), and Group C was less than Group A and Group B(P<0.05),Group B was less than Group A(P<0.05). Conclusion: 1. Hu IL-37 can inhibit KC proliferation in the lesions of psoriasis vulgaris. It also shows a dose response relationship within a certain concentration, The higher the concentration is, the more obvious the inhibition on proliferation is. 2. Hu IL-37 can inhibit the expression of IL-6, TNF-α and IFN-γ m RNA in KC in the lesions of psoriasis vulgaris. It shows a significant dose response relationship within a certain concentration, The higher the concentration is, the more obvious the inhibition is. |
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