The Influence Of Intestinal Lymphatic Ligation And Traditional Chinese Medicine In The Yangming Fushi Induced Kidney And Inteseine Injury Of Rat

Objective: observe the influence of Intestinal lymphatic ligation in the Yangming Fushi induced kidney and intestine injury rat;With free anthraquinones interpose Yangming Fushi rats,to study its impact on the kidney and intestine injury rats.Methods :(1) male Sprage-Dawley(SD) rats were randomly divide into:the saline group, saline+intestinal lymph duct ligation group, Yangming Fushi model group and Yangming Fushi +intestinal lymph duct ligation group.The Yangming Fushi model:The sever acute pancreatitis was induced by retrograde infusion of bilio-pancreatitis duct with 3.5% sterile sodium tanrocholate solution.Isolated mesenteric lymph duct,silk ligated prepare saline+intestinal lymph duct ligation group and Yangming Fushi +intestinal lymph duct ligation group.Respectively,6h,12 h,24h death the rats draw kidney and intestine tissue,part of tissue homogenates,partially embedded,sliced and HE staining.enzyme-linked immunosorbent assay(ELLSA) measured the levels of tumor necrosis factor-a(TNF-α),interleukin-1(IL-1) in kedney and intestine tissue homogenate enzyme myeloperoxidase detect the level of myeloperoxidase(MPO),Light microscopy of kidney and intestine histopathological changes.(2)male SD rats were randomly divided into:saline group, Yangming Fushi model group and Yangming Fushi+free anthraquinone of rhubarb group;Free anthraquinone of rhubarb(20g/kg) was administrated affusedly down alimentary canal from mouth to stomach with 2ml at 2 hours before and after the inducement of Yangming Fushi model. Respectively,6h,12 h,24h death the rats draw kidney and intestine tissue,Methods and indicators with a method(1).Results:(1)kidney tissue homogenates :compared with Yangming Fushi model group,the levels of TNF-α, IL-1 and MPO in 6h、12h、24h in Yangming Fushi +intestinal lymph duct ligatioon group were significantly decreased(P<0.05).the levels of serum TNF-α、IL-1 in 12h、24h in yangming fushi +free qnthraquinone of rhubarb group were significantly decreased(P<0.05).the level of MPO in 6h was decreased(P<0.05).Light microscopy histological changes in the kidney of rats,kidney injury in rats significantly improved in both yangming fushi +intestinal lymph duct ligation group and yangming fushi +free anthraquinone of rhubarb group.furthermore the inflammation of them is reduced than yangming fushi group.(2)bowels tissue homogenates :compared with Yangming Fushi model group,the levels of intestinal tissue homogenate TNF-α, IL-1 and MPO in 6h、12h、24h in Yangming Fushi +intestinal lymph duct ligatioon group were elevated,but the difference is not obvious;the levels of TNF-a、IL-1 in 12h、24h in yangming fushi +free qnthraquinone of rhubarb group were significantly decreased(P<0.05).the level of MPO in 6h was decreased(P<0.05).Light microscopy of intestinal pathological changes,compares with the yangming fushi model group,yangming fushi +intestinal lymph duct ligation group was no difference,intestinal injury in rats significantly improved in yangming fushi +free qnthraquinone of rhubarb group.Conclusions:(1)The intestinal lymph duct ligation can protect the pathology of kidney injury,but no treatment effect on intestinal tissue.(2)The free anthraquinone of rhubarb can protect the pathology of kidney and intestine damage in the Yangming Fushi rats.