The Expression And Significance Of Rnd3 And Eps8 In Adenomyosis

Objective:We detected the expressions of Rnd3 and Eps8 which were related cell migration in ectopic endometrial, eutopic endometrium of adenomyosis(AM) and control endometrium from uterine myoma by immunohistochemistry. We also analyzed the mutual relationship of Rnd3,Eps8 and cell migration in AM, and explored the roles of these proteins in the development process of AM. Methods:1.We collected ectopic endometrium and eutopic endometrium specimens from 32 patients who had been resected uterus because of the adenomyosis in this study, designed as ectopic group and eutopic group. Meanwhile, 32 cases of control group were selected from the patients because of myoma and without other uterine tumors. All samples were collected from the department of obstetrics and gynecology in Affiliated Hospital of Luzhou Medical College. 2. All the patients to participate in this study had not been taken hormone medication and accepted the treatment of uterine adenomyosis at least half a year before this operation. All the pathological diagnoses were confirmed by two high qualification pathologists. The expressions of Rnd3 protein and Eps8 in these three groups of endometrial tissue were measured by immunohistochemistry. We detected the mean optical density of the positive cells by professional image analysis system 6.0, and analyzed the levels of expression and theircorrelation both in the three groups. 3.Statistical analysis: The experiment data was analyzed by SPSS 17.0 software, and the significant level was defined as P<0.05. Results: 1. the expression of Rnd3 protein in ectopic endometrium of AM was significantly decreased, compared with control endometrium and eutopic endometrium,(P<0.05, P<0.05).The expression of Rnd3 in eutopic endometrium was lower than that in control group(P>0.05). 2. the expression of Eps8 in ectopic endometrium was obviously higher than eutopic endometrium and control endometrium(P<0.05,P<0.05). The expression of Eps8 in eutopic endometrium was slightly higher than the control group(P<0.05). 3.There was a negative correlation between Rnd3 and Eps8 both in ectopic group(r=-0.517, P=0.002<0.05)and eutopic group(r=-0.550, P=0.001<0.05). There was no correlation between Rnd3 and Eps8 in control group(r=-0.120, P=0.513>0.05). Conclusion:1.The significant down-regulation of Rnd3 and obvious raised of Eps8 in the ectopic endometrium group and eutopic endometrium group of AM,which suggested that the abnormal expression level of Rnd3 and Eps8 may have contributed to the development of endometrial and proliferation in ectopic endometrium. Consequently,ectopic implantation and growth could be achieved easily. This is one of the important cause leading to the adenomyosis.2.In the three groups, the expression of Rnd3 was lowest in ectopic group and the expression of Eps8 was highest in ectopic group,which further suggested that the negative feedback or antagonist relationship in ectopic group was most obvious. The imbalance of Rnd3 and Eps8 may be a cause of the growth of ectopic endometrial cell.It also could be jointly promote the development of adenomyosis.However,the specific mechanism remains to be further study.3.There was a negative correlation between Rnd3 and Eps8 both in ectopic group and eutopic group, which suggested there may be a antagonist relationship or negative feedback between Rnd3 and Eps8.