| ObjectiveTo explore the efficacy of kelpmierogelation(KMG) Containing Nordy in treating rabbit VX2 hepatic carcinoma.MethodSelecting 50 New Zealand white rabbits, and using the method of cutting the belly open to prepare VX2 carcinoma model.1. Intervention treatment: After HCC model was confirmed by enhanced CT scan two weeks later, the 50 rabbits were randomly divided into five groups:physiological saline group(perfused with 1ml/kg NS), TACE group(perfused with 1mg/kg KMG and 1 mg/kg 5- Fu), KMG group(perfused with 1mg/kg KMG), Nordy group(perfused with 1mg/kg Nordy),Nordy-KMG group(perfused with 1mg/kg KMG containing Nordy).2. Treatment after intervention: Observing the general situation after operation; observing the dynamic changes of Alanine aminotransferase(ALT), aspartate aminotransferase(AST) and creatinine(Cr) at 1 th day before treatment and 1th, 3th, 7th day after;observeing the CT images,measuring the maximum and minimum diameter of the tumor, calculating the growth rate of tumor volume, observing the blood supply, necrosis and metastasis of tumor with enhanced CT scan, the number and rate of metastasis cases should be calculatedr; rabbits were sacrificed at 14 th day after treatment, and cutting non-necrotic area surrounding the tumor and surrounding liver tissue, then fixing them with 4% formaldehyde, doing HE staining to research the microscope findings and immunohistochemical staining to investigate expression of the vascular endothelial growth factor(VEGF), generating of microvessel density(MVD) after interventional procedures.Result1. General situation after treatment After treatment, the rabbits in five groups had a significantly reduction of transient activities, listlessness,anorexia and unresponsive, especially in TACE group, KMG group and Nordy-KMG group.2. Liver and kidney function The AST and ALT rose up significant at 1thday and 3th day after treatment in TACE group, KMG group and NordyKMG group, and no significant difference among the three ones, but a significant difference between physiological saline group, Nordy group(P<0.05). The two indexs decreased to the level pre-treatment at 7thday after treatment. The change of Cr has no significant difference among each group(P>0.05).3. Imaging findings Rabbit VX2 carcinoma showed round low density on the abdomen CT scan,and ring enhancement on the enhanced CT scan,but the center was low density. before interventional treatment,the arterial DSA showed an increase in tumor blood vessels, thickening, tortuous, some were typical "hold the ball like" change,the essence DSA showed the tumor was "nodular" staining; most of the tumor staining subsided after embolization.The tumor volume growth rate of TACE group( 131.82 ±47.90 %), KMG group( 176.76 ± 36.94 %) and Nordy-KMG group( 118.10±31.64%) were less than physiological saline group(460.94±112.14%)and Nordy group(388.00±98.83 %), The difference was statistically significant(P<0.05). The transfer rate of TACE group(29.6%), KMG group(40.0%) and Nordy-KMG group(22.2%) were less than physiological saline group(88.9%), Nordy group(80.0%).4. Pathology The HE staining showed tumor nuclei large, shaped apparent disorder, central large tumor tissue degeneration, necrosis and the structure is unclear.Immunohistochemistry shows VEGF protein levels in each group varies, the positive rates were: 70%, 70%, 80%, 60% and 50%, MVD was55.45 ± 6.16,63.17 ± 6.42, 64.59 ± 7.68,54.53 ± 6.23, 44.67 ± 8.22. VEGF expressing cells have the lowest percentage of total cells in Nordy-KMG group and the highest percentage in KMG group.The MVD expression in Nordy-KMG group was least, compared with TACE and KMG group, the difference was statistically significant(P = 0.00); physiological saline group compared with Nordy group, there has no statistically significant difference(P = 0.16). TACE Group compared with KMG group, the difference was no statistically significance(P = 0.72).Conclusion1. Nordy-KMG is is a safe and effective, low toxicity of the drug microspheres, which has broad application prospects in anti-tumor therapy.2. Nordy-KMG can could inhibit tumor growth, reduce tumor recurrence and metastasis.3. Nordy-KMG could inhibit tumor angiogenesis Obviously. |
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