| Objective: The incidence rate of diabetes mellitus is increasing day by day and diabetes mellitus own a great variety of complication up to now. One of them that would lead to a lower standard of living is the Female sexual dysfunction. The reason of female diabetic Mellitus patients’ vaginal lubrication decline has not been thoroughly investigated up to now. This examination would investigate the difference of Water channel proteins’(aquaprins proteins, AQPs)expression in vaginal tissues between diabetic Mellitus rat and normol rat to explain the relationship of subgroup AQPs and hyperglycemia, figure out the possible influence of AQPs’ expression in female diabetic Mellitus patients, and provide new way of treatment for sexual dysfunction in women whom troubled with diabetes mellitus. Methods: Female Sprague-Dawley rats(6 weeks old and healthy)(n=24) were adaptive feeding for 2 weeks, then they were randomly distributed into 4 experimental groups after the adaptive feeding: Group A(4 week diabetic mellitus group, N = 6), Group B(4 week nondiabetes control, N = 6), Group C(6 week diabetic mellitus group, N = 6), Group D(6 week nondiabetes control, N = 6).All of the rats were Fasted for 12 hours before the models of diabetic rat were established.The method of how to establish diabetic rat is both Group A and Group C were received 1% STZ single intraperitoneal injection 55mg/kg, the rest of the animal were received the same amount of citric acid buffer solution by intraperitoneal injection.Blood glucose more than 16.8mmol/L, the decrease of body weight, polydipsia and polyuria suggesting that the model of diabetic rat was successful. Weight of all about the animals were regular detected after fasting 12 hours without restriction of drinking water, as well as Blood Sugar were regular detected too. Determinate the level of estradiol of serum, the weight of vaginal lubricating, the expression site were analyzed by immunohistochemistry and we use Western Blot to analyze the amount of AQPs. Results: The serum concentrations of glucose of 4 week diabetic mellitus group(29.07 ± 3.67mmol/l) and 6 week diabetic mellitus group(28.15 ± 5.92mmol/l) were significantly increased against 4 week nondiabetes control(5.67 ± 1.11mmol/l) and 6 week nondiabetes control(5.63 ± 0.92mmol/l)(P<0.05). The body weight of 4 week diabetic mellitus group(192 ± 6.07g) and 6 week diabetic mellitus group(198 ± 3.83 g) were significantly decreased against 4 week nondiabetes control(249 ± 7.62g) and 6 week nondiabetes control(267 ± 11.41g)(P<0.05). The ratio which is the weight of vaginal secretion after electrically stimulate pelvic nerve and the weight of vagina of female rats is vaginal lubrication. The vaginal lubrication of 4 week diabetic mellitus group(2.12 ± 1.41) were significantly decreased against 4 week nondiabetes control(4.21 ± 0.86)(P<0.05), and the vaginal lubrication of 6 week diabetic mellitus group(2.64 ± 0.88) were significantly decreased against 6 week nondiabetes control(4.41 ± 0.77)(P<0.05). All of groups have no significant diversity in the plasma levels of estrogen.Immunohistochemistry staining indicated that AQP0 was mainly expressed in the cytoplasm of epithelial cells of vagina, western blot analysis revealed that the relative optical density of AQP0 expression in the vaginal tissues of 4 week diabetic mellitus group(29.74 ± 7.21) were significantly decreased against 4 week nondiabetes control(116.62±3.21)(P<0.05), and the the relative optical density of AQP0 expression in the vaginal tissues of 6 week diabetic mellitus group(31.56 ± 7.18) were significantly decreased against 6 week nondiabetes control(102.59 ± 9.59)(P<0.05). Immunohistochemistry staining indicated that AQP5 was mainly expressed in the vascular endothelial cells and stromal cell nucleus of vagina, western blot analysis revealed that the relative optical density of AQP5 expression in the vaginal tissues of 4 week diabetic mellitus group(36.31 ± 2.53) were significantly decreased against 4 week nondiabetes control(100.62 ± 2.76)(P<0.05), and the the relative optical density of AQP5 expression in the vaginal tissues of 6 week diabetic mellitus group(35.39 ± 3.42) were significantly decreased against 6 week nondiabetes control(106.14 ± 5.39)( P < 0.05). Immunohistochemistry staining indicated that AQP6 was mainly expressed in the vaginal blood capillary and vein endothelial cells of vagina, western blot analysis revealed that the relative optical density of AQP6 expression in the vaginal tissues of 4 week diabetic mellitus group(68.42 ± 5.48) were significantly decreased against 4 week nondiabetes control(106.78 ± 3.64)(P<0.05), and the the relative optical density of AQP6 expression in the vaginal tissues of 6 week diabetic mellitus group(67.42 ± 1.97) were significantly decreased against 6 week nondiabetes control(110.22 ± 1.47)(P<0.05). Western blot analysis revealed that the relative optical density of AQP10 expression in the vaginal tissues of 4 week diabetic mellitus group( 24.63 ± 1.14) were significantly decreased against 4 week nondiabetes control(84.34 ± 4.32)(P<0.05), and the the relative optical density of AQP10 expression in the vaginal tissues of 6 week diabetic mellitus group(28.49 ± 2.02) were significantly lower than 6 week nondiabetes control(87.7 ± 5.72)(P<0.05). Immunohistochemistry staining indicated that AQP11 was mainly expressed in the vaginal blood capillary and vein endothelial cells of vagina, western blot analysis revealed that the relative optical density of AQP11 expression in the vaginal tissues of 4 week diabetic mellitus group(26.86 ± 2.9) were significantly lower than 4 week nondiabetes control(93.27 ± 12.25)(P<0.05), and the the relative optical density of AQP11 expression in the vaginal tissues of 6 week diabetic mellitus group(27.15 ± 2.44) were significantly decreased against 6 week nondiabetes control(94.27 ± 10.2)(P<0.05). Western blot analysis revealed that the relative optical density of AQP12 expression in the vaginal tissues of 4 week diabetic mellitus group(29.98 ± 8.41) were significantly lower than 4 week nondiabetes control(94.57 ± 10.47)(P<0.05), and the the relative optical density of AQP12 expression in the vaginal tissues of 6 week diabetic mellitus group(34.4 ± 11.2) were significantly decreased against 6 week nondiabetes control(91.37 ± 19.67)(P<0.05).Conclusion: The lubrication of the vagina diabetes mellitus rats was significantly reduced against that in the nondiabetes control rats. The display about those water channel proteins such as AQP0, 5, 6, 10, 11, 12 in vaginal tissue of rats were negatively correlated with blood glucose. The reduce of vaginal lubrication may be related to the lower expression of those water channel proteins such as AQP0, 5, 6, 10, 11, 12. |
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