The Bleaching Efficiency And Bio-safety Assessment Of Plasma Activated Water Assisted By Low Concentration Hydrogen Peroxide

Pure white teeth are vital to one’s appearance, and mostly of people were unsatisfied with the color of their teeth. Therefore, tooth bleaching has become a popular esthetic service in dentistry, hydrogen peroxide (HP) plays a guide function in the whole tooth bleaching activities for more than a century. Existing techniques is varied and each have their own strengths, however, none of them for optimum whitening can achieve the goal of considering both efficiency and safety. As a new method of tooth whitening, non-thermal plasma has been reported to have certain effective for tooth whitening and the temperature of tooth during treatment is acceptable according to the studies of home and abroad. The excellent tooth whitening properties and biosecurity make it hopeful for clinical practice. However, researches focused on the limitation of the device of plasma. PAW (Plasma Activated Water, PAW) can be considered as a convenient disinfection solution that is activated by plasma discharges for a period of time. Because PAW also contains variety reactive oxygen species, we blocked out our ideas for the instead of plasma by PAW for tooth bleaching to increase the feasibility and acceptance of plasma in the field of tooth bleaching.Objective:Investigate the whitening properties and biosecurity of PAW assisted by low concentration hydrogen peroxide (H2O2).Methods:1, Thirty-Six bovine central incisor crowns were stained by black tea, whitening properties of PAW alone,3% H2O2 alone,30% H2O2 alone and PAW+3% H2O2 are investigated by CIE Lab spectrophotometric measurements at baseline,15 min,30 min,1 h,2 h,3 h of bleaching treatment respectively.2, Evaluate the oxidation reduction potential (ORP) of the PAW or PAW+3% H2O2 by ORP meter with an ORP probe. FTIR was used to analyze the generation of·OH free radicals of different groups.3, Histologic study was conducted to evaluate the biosecurity of the all above bleaching agents by topical application on hamster cheek pouch.Result:1, The results showed that PAW+3% H2O2 was significantly more effective than the PAW or 3% H2O2 alone for tooth bleaching (p< 0.01), and PAW+3% H2O2 had equal or better effect on tooth bleaching than 30% H2O2 for 2h treated.2, Hydroxyl free radicals (·OH) considered to be one of the key agents during the tooth-whitening process. Plasma activated species in PAW had increased the ORP and content of hydroxyl radicals in the mixed solution of PAW and low concentration of hydrogen peroxide.3, Histologic study indicated that PAW+3% H2O2 was mild and less-stimulated compared to 30% H2O2, which could avoid the chemical burns of the mucous membrane.Conclusion:Activated water by non-thermal plasma (PAW) mixed with 3% H2O2 can be an effective and safe method for tooth bleaching.