Study On ENOS Gene T786C、G894T Polymorphism And Susceptibility Of Intracranial Aneurysm

Objective:Intracranial aneurysm is an abnormal bulge of formation resulted from the cerebral artery abnormal enlargement of arterial wall under factors like inflammatory factors, hemodynamic factors. Rupture of intracranial aneurysms can cause aneurysmal subarachnoid hemorrhage, leading to high mortality and morbidity. At present, the pathogenesis of intracranial aneurysms is not yet clear, a more consistent view is determined by both environmental and genetic factors results. The association between T786C and G894T endothelial nitric oxide synthase (eNOS) gene polymorphisms and susceptibility of intracranial aneurysm(IA), which included rupture or non-rupture IA, has been widely reported, but the results are still controversial. Therefore, a meta-analysis is necessary to investigate the association of them.Methods:In this meta-analysis, Hardy-Weinberg equilibrium(HWE) was performed to test in controls. The odds ratio (OR) and 95% confidence interval (95% CI) were selected to assess the strength of association. Funnel plots, Begger’s test and Eegg’s linear regression test were used to evaluate the small study biases. There were 1082 cases and 1219 controls for T786C polymorphism and 495 cases and 617 controls for G894T polymorphism.Results:Overall, T786C polymorphism was significantly associated with susceptibility of IA under allele contrast model (OR=1.22,95%CI=1.04-1.44, P=0.014) and dominant model (OR=1.28,95%CI=1.05-1.56, P=0.013). In the subgroup analysis of ethnicity, a significant association was also presented in Asian population under allele contrast model (OR=1.28,95%CI=1.05-1.58, P=0.016) and dominant model (OR=1.30,95%CI=1.04-1.63, P=0.021), but not in Caucasian population under all five models:comprised allele contrast (C vs T); homozygous comparison (CT vs TT); dominant (CC+CT vs TT); recessive (CC vs CT+TT); codominance (CC+TT vs CT). However, there was no significant difference between G894T polymorphism and susceptibility of IA under all the five models:comprised allele contrast (T vs G); homozygous comparison (TG vs GG); dominant (TT+TG vs GG); recessive (TTvs TG+GG); codominance (GG+TT vs TG).Conclusions:The meta-analysis results showed that eNOS T786C polymorphism is significantly associated with intracranial aneurysm susceptibility in all included studies, especially in Asian population. It is suggest that eNOS T786C gene polymorphism is a valuable risk factor to predict IA susceptibility.