The Comparison Of Polymerase Chain Reaction And Bacteria Culture In Peritoneal Dialysis Related Peritonitis

Objective Application of polymerase chain reaction(PCR) detection in patients with peritoneal dialysis related peritonitis(PDRP) through abdominal fluid specimens of pathogenic bacteria distribution, which compared with the traditional training focus is to explore the clinical application of PCR in the PDRP bacteria detection value.Methods 1.According to the data collected from January 2014 to December 2014 for PDRP check affiliated hospital of An Hui Medical University to drain fluid specimens, 40 patients were divided into two, a specimen of traditional bacterial culture and drug sensitive test, another specimen under 80°C refrigerator for subsequent PCR method to detect. 2.Basis on the literature and refer to the same period of the traditional bacterial culture results, then establish the experimental detection of 17 experimental bacteria. 3.According to the bacterial 16 S r RNA gene design universal primers, and reference to design the specific primers, 17 kinds of experimental bacteria on the clinical specimens after extracting DNA real-time fluorescence PCR amplification. Standard and setting up the experimental bacteria strains DNA extract as a positive control, sterile double steaming water as a negative control.4.The PCR results comparing with conventional training results, using SPSS19.0 statistical software for data analysis of clinical data.Results 1.The traditional bacterial culture results showed that positive rate was 65.00%(26/40) in specimen of 40 cases,gram-positive strains accounted for 69.23%(18/26).main species are: epidermis staphylococcus(13.89%), hemolysis staphylococcus(11.11%), escherichia coli(11.11%), streptococcus viridans(8.33%).2.From the patient’s clinical statistics,white blood count and blood CRP of the patients with positive culture test is obviously higher than the negative ones.Almost all patients were abdominal pain and cloudy peritoneal fluid.Six of the positive group had past history of infection,while the negative group was three,which was no statistically significant.3.The PCR detection results showed that 40 patients specimen total positive rate was 82.50%(33/40), 2 cases of positive samples have not been measured specific strains;The main strains no difference with normal training results.4.With bacterial culture as the gold standard, the PCR technology of PDRP pathogenic bacteria detection sensitivity of 96.15%, specificity of 42.86%, and the detection positive rate is significantly higher than ordinary culture method.5.PCR technology for detecting pathogenic bacteria can produce results within 24 hours,report positive results in the traditional bacterial culture would take 66-115 hours(77.88± + 15.53 hours), it is significantly longer than PCR.Conclusion Compared with traditional bacteria culture method, PCR method is a more sensitive simple and quick. Bacteria detection using PCR technique is of clinic applied value in PDRP.