Prostate Cancer In Urine And Peripheral Blood PSAmRNA And DD3mRNA Expression And Clinical Value

Objective: Through the comparison and analysis of research and clinical value of PSAm RNA and DD3 m RNA expression in prostate cancer patients with urine and peripheral blood, and PSAm RNA and DD3 m RNA expression and the relationship between each other. Providing multiple targets and many markers for early diagnosis and evaluation of specific therapeutic effect of prostate cancer.Methods: Through fluorescence quantitative reverse transcription polymerase chain reaction(FQRTPCR)detection method, content of normal people in patients with benign prostatic hyperplasia were measured in 34 patients with confirmed diagnosis of prostate cancer in 73 cases, confirmed and 23 patients with chronic prostatitis patients and 20 healthy physical examination of urine and peripheral blood Ps A m RNA and DD3 m RNA.Results: 1 The total concentration of RNA obtained for 178 ug / ml, the extraction amount of totalresearch is about 3.9 UG, are in line with the provisions of this study further examinationstandard. At the same time, by UV spectrophotometer, determination of RNA respectively in260 nm and absorbance wavelength of 280 nm, the ratio of system analysis, between A260 and A280 results were found, the ratio between them is in the range of 1.6-2.0.2 Acquisition of lug by reverse transcription and synthesis methods of c DNA, through GAPDHwith reference to the standardization of the PCR amplification, results show that the amplifiedfragment is 315 bp in the experiment.3 PSAm RNA in the prostate cancer group, prostatic hyperplasia and prostatitis group object of study group were significantly higher than those in the urine content of normal people group PSAm RNA in the urine(Z = 5.364, P=0.026; Z=4.209, P=0.018; Z=3.196, P=0.040;). At the same time, the content of PSAm RNA in prostate cancer group of subjects in the urine was significantly higher than that of PSAm RNA in benign prostatic hyperplasia and prostatitis groupgroup of subjects in the urine(Z =7.186, P=0.009; Z = 6.175, P=0.011;), while the content of PSAm RNA in benign prostatic hyperplasia group of subjects in the urine was much higher than that in PSAm RNA group of prostatitis objects in the urine(Z=9.163, P=0.002).4 In the four group of subjects only prostate cancer group object of study population found in blood with DD3 m RNA expression, the other three groups of subjects showed no, in prostatedisease group and normal group can be different in the peripheral blood of the discovery of PSAm RNA, but the content from high to low is the normal population of prostate cancer group >> chronic prostatitis prostate hyperplasia group group > group, through the testing method to take Mann Whitney U, a further two two comparison: content, found in prostate cancer, benign prostatic hyperplasia and prostatitis group group group research object in the peripheral blood PSAm RNA content were significantly higher than those in normal group PSAm RNA of the peripheral blood(Z=4.163, P=0.036; Z=8.217, P=0.006; Z=9.006, P= 0.003).5 Determination of the content of PSAm RNA in the prostate cancer groups of subjectsperipheral blood was significantly higher than that of benign prostate hyperplasia and prostatitisgroup object of study group content of PSAm RNA of the peripheral blood(Z=5.203, P=0.025;Z=7.139, P=0.012;), while the content of prostatic hyperplasia group of subjects in peripheral blood was significantly higher than that of PSAm RNA. Prostatitis was the research object the content of PSAm RNA of the peripheral blood(Z=3.189, P=0.043).6 The median DD3 m RNA prostate cancer research object in urine samples of 329.79 X102,while numerical in benign prostatic hyperplasia in populations of the above items 4.40x1027 If the diagnostic cutoff value is set to 0.100 cases, measured in urine of DD3 m RNA in the diagnosis of prostate cancer had a sensitivity of 0.728, specificity of 0.713; if the diagnosis oftruncation in peripheral blood of DD3 m RNA is set to be larger than 0 of the cases, the resultshave no peripheral blood DD3 m RNA had a sensitivity of 0.289 in the diagnosis of prostatecancer, with 1 specificity.8 The positive rate was positively correlated with content of peripheral blood DD3 m RNA, prostate cancer group objects of urine DD3 m RNA, peripheral blood PSAm RNA, urine PSAm RNA and serum PSA and prostate cancer(P<0.05).Conclusions:1 The high specificity of DD3 m RNA in the diagnosis of prostate cancer than that in specific PSAm RNA and PSA in serum in the clinical, DD3 m RNA can be used as a specific marker for diagnosis of prostate cancer;2 Expression of DD3 m RNA in urine of higher in the diagnosis of prostate cancer than the value of the expression of DD3 m RNA in peripheral blood of diagnosis;3 The positive rate was positively correlated with the content of peripheral blood DD3 m RNA,urine DD3 m RNA, peripheral blood PSAm RNA, urine PSAm RNA and serum PSA and prostate cancer.