Protective Effect On The Focal Cerebral Ischemia/Reperfusion Injury And Antithrombotic Activity Of The Prescription Of Salvia Miltiorrhiza Bge., Astragalus Membranaceus (Fisch.) Bunge.,Pueraria Lobata (Willd.) Ohwi.and Other Medical Materials

Cerebral ischemia/reperfusion (I/R) injury resulted from the treatment of ischemic cardiovascular and cerebrovascular diseases threatens human health with high incidence rate of death and disability. Thrombosis is one of the main causes of many ischemic cardiovascular and cerebrovascular diseases. Traditional Chinese medicines have protective effects on cardiovascular and cerebrovascular diseases through multidirection and multiple targets. Salvia miltiorrhiza Bge., Astragalus membranaceus (Fisch.) Bunge., Pueraria lobata (Willd.) Ohwi. are individually used in many compatibility prescriptions for the treatment of ischemic cardiovascular and cerebrovascular diseases, however there is no experimental report about the protective effect on cerebral I/R injury and antithrombotic activity of the prescription.Cerebral I/R injury in rats was induced by middle cerebral artery occlusion. The protective effect of the compatibility of Salvia miltiorrhiza Bge., Astragalus membranaceus (Fisch.) Bunge., Pueraria lobata (Willd.) Ohwi. on rats’ cerebral I/R injury were estimated by neurological defect scoring and TTC staining. The levels of MDA, CAT, SOD, iNOS and TNOS in serum were measured by biochemical assay kits. Meanwhile, histological structures of the cortex of ipsilateral ischemic cerebral hemisphere in rats were observed by Nissl staining. The Caspase-3 protein content in hippocampus and the cortex was detected by immunohistochemistry. Additionally, Bax and Bcl-2 protein expressions in the injured brain were determined by Western blot. It was found that this prescription could significantly improve neurological dysfunctions, lessen the cerebral infarction volume, increase surviving neurons, lower MDA content, up-regulate SOD and CAT levels, down-regulate iNOS and TNOS levels, restrain the expression of Caspase-3 positive protein and alleviate the Bax and Bcl-2 protein expressions significantly. The results suggested that this prescription had the protective effect on cerebral I/R damage in rats. The protective mechanisms are associated with its properties of anti-apoptotic and anti-oxidant.Ice water bath and subcutaneous injection of norepinephrine and bovine serum albumin were both used to establish the acute blood stasis rat model. After electrical stimulation on the carotid artery, the antithrombotic effect and mechanism of the prescription was evaluated by measuring the thrombus’length, wet weight and histological features, the anticoagulant activities and relevant blood biochemical index. Our results showed that the treatment of this prescription was able to reduce thrombus’length and wet weight, prolong APTT, TT and PT, decrease FIB content, down-regulate serum TXB2 and plasma ET level, up-regulate serum 6-keto-PGF1α level and plasma eNOS level.It suggests that the prescription of Salvia miltiorrhiza Bge., Astragalus membranaceus (Fisch.) Bunge., Pueraria lobata (Willd.) Ohwi. possesses the protective effect on cerebral I/R injury in rats, antithrombotic effect on blood stasis rats, which provides a theoretical basis for the treatment of cardiovascular and cerebrovascular diseases.