The Association Of The MMP-2 Gene Polymorphism With The Risk Of Colorectal Cancer

Objective: Discussion on matrix MMP-2 rs2285053C/T、rs243865C/T single nucleotide polymorphism and susceptibility of colorectal cancer.To provide the scientific basis for early diagnosis and individualized treatment of colorectal cancer.Methods: Between February 2013 and February 2015 in the Fourth Affiliated Hospital of Hebei Medical University, 102 cases of colon cancer patients, 138 cases of rectal cancer patients and 147 healthy people were chosen to participate in the case-control study. We collected from the participants matching the conditions of the experiment, with their informed consent, venous blood of 5 ml, as well as their age, gender, smoking, drinking, family history and other information. The proteinase K digestion-saturated nacl salting out method was applied to extract the DNA in white blood cells, and the Polymerase Chain Reaction-ligase Detection Reaction(PCR-LDR) was used to detect MMP-2 gene and analyze two SNP loci alleles and genotype of rs2285053C/Tandrs243865C/T.SPSS Ver13.0 software kit(SPSS Company in New York, Chicago, Illionis, USA) was used for statistical analysis of the experimental result. T-test was conducted on age differences between rectal cancer group, colon cancer group and health control group; chi-square test was carried out on gender differences between the two groups and the distribution of allele frequency and genotype frequency distribution; Hardy-Weinberg equilibrium analysis was performed by comparing the observed and expected genotype; unconditional logistic regression method was applied to calculate the odds ratio(OR) of relative risk degree with 95% confidence interval(CI); and EH software and 2LD software were adopted to analyze the two SNPs of MMP-2 gene rs2285053C/Tandrs243865C/T, with P<0.05 as the dividing line of a significant difference.Results:1 The genotype frequency distribution of rs2285053C/Tandrs243865C/T met the Hardy-Weinberg equilibrium in the control group(P> 0.05).2 The analysis of correlation between MMP-2 rs243865C/T polymorphism and the risk of CRCThe frequencies of alleles A and G on MMP-2 rs243865C/T in the rectal cancer group were 90.9% and 9.1% respectively, while in colon cancer group they were 91.7% and 8.3%, and in control group 85.4% and 14.6%. There were statistical differences between the rectal cancer group and the control group, or between the colon cancer group and the control group(P was 0.049and0.034 in both cases). In the rectal cancer group, the frequencies of genotypes C/C,C/T,TT were 83.3%,16.4%,1.5% respectively,while they were 84.3%,14.7%,1.0% in colon group, and 72.8%,28.2%,2.0% in control group. There were statistical significant differences between the rectal cancer group and the control group or the colon cancer group and the control group(P <0.05). Comparing with those with genotype(CC+CT), the people with genotype CC suffer a higher risk of colorectal cancer(OR=1.869,95% CI=1.050-3.327;OR=2.009,95%CI=1.054-3.832).3 The analysis of correlation between MMP-2 2285053C/T polymorph- ism and the risk of CRC.The frequencies of the alleles C and T on MMP-2 2285053C/T in the rectal cancer group were 76.8% and 23.2% respectively, while in colon cancer group they were 81.9% and 18.1%, and in control group 79.6% and 20.4%. There were no statistical difference between the rectal cancer group and the control group, or between the colon cancer group and the control group(P were 0.536 or 0.421 respectively). In the rectal cancer group, the frequencies of the CC,CT,TT genotypes were 56.5%,40.6%,2.9% respectively, while they were 66.7%,32.3%,1.0% in colon group, and 63.2%,32.7%,4.0% in control group. And there was no statistical difference between the rectal cancer group and the control group, or between the colon cancer group and the control group(P were 0.246 and 0.584 respectively). Therefore, no statistical difference existed to support the argument that Comparing with those with genotype(CT+CT), people with genotype CC have a higher risk of developing rectal cancer(OR=0.765, 95%CI=0.469-1.214); nor was there any statistical difference to support the argument that comparing with those with genotype CC, people with genotype(CT+TT) shall suffer no higher risk of developing colon cancer(OR=1.161, 95%CI=0.683-1.975).Conclusion:1 The gene rs243865C/T polymorphism of may be associated with genetic susceptibility to colorectal cancer in the shijiazhuang populations. The Allele C may be associated with genetic susceptibility to colorectal cancer in the Shijiazhuang populations. the CC genotype increased the risk of colorectal cancer.2 The gene polymorphism of MMP-2 rs2285053 was not associated with genetic susceptibility to colorectal cancer in the Shijiazhuang populations.3 The haplotype rs2285053C-rs243865 C could Increase the incidence of colorectal cancer.