The Expression And Significance Of Regulator Of G-protein Signaling 5 In Esophageal Carcinomas

Objectives: The Regulator of G-protein signaling 5(RGS5) is a factor recently found to be associated with tumor angiogenesis, and little has been reported in esophageal carcinomas. The objectives of this study are to observe the expressions of RGS5 in both esophageal tumor tissues and tissues within 5cm to tumors(normal tissues), to compare the differences of RGS5 expression between tumor and normal tissues, to explore the relationship among RGS5, the clinical pathological features and tumor microvascular density(MVD), and hope to provide theoretical support for understanding the development of esophageal carcinomas.Methods: 1. The specimens from 46 patients with esophageal carcinomas were divided into two groups. The experimental group included the tumor tissues of esophageal carcinomas with invasion of cancer cells confirmed by microscopic pathology. The control group included the normal tissues within 5 cm to tumor tissues with invasion of cancer cells ruled out by microscopic pathology. 2. The expression of RGS5 were detected by both the Real-time PCR and immunohistochemical staining in both groups. And then the relationship between the RGS5 expression and the clinical pathological features of the patients was analyzed. 3. CD34 in the tumor tissues which served as an index of tumor microvascular density were detected by immunohistochemical staining, and the relationship between the RGS5 expression and the MVD value were explored.Results: 1. The expressions of RGS5 were significantly increased in the tumor tissues than those in the normal tissues in which there were barely expressions of RGS5(P<0.05). 2. The expressions of RGS5 were significantly different among the tumor differentiation degree subgroup(P<0.01), while not related with other clinical pathological subgroups such as age, gender, the diameters of tumors, clinical stages(P>0.05). 3. The tumor microvascular density was significantly increased in tumors with lower RGS5 expressions than that with higher RGS5 expression(P<0.05).Conclusions: 1. The expressions of RGS5 were significantly increased in esophageal carcinomas and perivascular cells compared with the few expressions in normal tissues.2. The expression levels of RGS5 in tumor tissues was related to tumor differention degree, but not related to gender, age, diameters of tumors, or clinic stages. 3. The expressions of RGS5 were inversely related to the tumor microvascular density. Our study indicated that RGS5 were related to tumor differentiation and angiogenesis, and intervention to RGS5 might affect tumor differentiation degree and angiogenesis.