| The hypoxia in tumor tissue is a main feature of microenvironmentof tumor growth. Though there’s hypoxia incubator for sale abroad, manylaboratories do not purchase it for its high price. Some domesticresearchers have set up several hypoxia models, however, most of themneed manual operation and can not monitor oxygen concentrationdiversity constantly in the incubator, for which we have used thesingle-chip computer technique to develop the system of cells trainingunder hypoxic and supply a stable culture system for the research ofhypoxia in cells. We choose PIC16F877a as a control unit to calculateanalog input through A/D module with successive approximation method,then A/D module will sends out level signal to relay, maintainingpermanent oxygen concentration by controlling open and close of thecylinder’s solenoid valve of oxygen and nitrogen. The establishedhypoxic culture system can regulate oxygen concentration varying from0%to95%by single-chip computer steering; this culture system cansatisfy the experimental requirements of different low oxygenconcentration.With this hypoxic culture system, we examined some indicators ofthe thymoma cell lines in hypoxic state. At present, the thymic tumor celllines of patients with myasthenia gravis with thymoma haven’t beenknown at home and abroad, there for, we have proceed primary culturethymic tumor tissue which collected from the operative patients whosuffered thymoma with MG, in order to approach the primary culturemethods of thymic tumor tissue and investigate the best condition andcomfortable culture medium of thymic tumor tissue and thymic epithelialtumor cell lines. After the primary culture became successful, weestablished a human thymic epithelial tumor cell line which was namedas Thy0517. This cell line provided a stabile cells model for the researchof molecular basis of thymoma invasion and the relationship of signaltransduction of MG. We have identified the origin of Thy0517cell line byimmunohistochemistry and immunocytochemistry, observed the featureof the cell line by inverted microscope and determine the time of cell doubling. We have also observed the fine structure of the cell byhematoxylin and eosin stain, calculated chromosome mode anddistribution by colchicine treatment, analyzed nude mouse tumorigenesisin vitro, and evaluated the invasive ability of the cell line with3%,5%,10%low-oxygen concentration by hypoxic culture system. |
