Povidone-iodine Volts Basic Research Of The Impact Of Nerve Tissue

Objective Studied in this paper through the animal experiment, the dilutions ofpovidone–iodine for the influence of the central nervous tissue, explore povidone–iodinein neurosurgery application security.Method60male Wistar rats weighing280±20g, the Wistar rats were randomlydivided into3groups: saline group,0.025%povidone–iodine solution,0.05%povidone–iodine solution. The Wistar rats simulated right frontal craniotomy surgery afteranesthesia, under the microscope cutting open the arachnoid, the area of exposed rats had0.5×0.5cm, separately,use saline,0.025%povidone–iodine solution,0.05%povidone–iodine solution rinse the brain tissue of Wistar rats5min, Again use saline rinse braintissue5min, bone window sealed up bone wax, suture of incision layer by layer,respectively execute the Wistar rats after rinsed3days, get the brain tissue and blood ofrats for related checklists, the lesion of rat brain tissue and its surrounding brain tissuedamage in general observation by the naked eye; by the light microscopy brain tissuesection lines using hematoxylin-eosin staining (HE) to observe the brain cells changes;by immunohistochemical staining observe the expression of NF-κB and AQP4;application of ELISA method for determination of serum neuron-specific enolase (NSE),matrix metalloproteinase (MMP-9) content; The above indicators for statistical analysis.Results Application of physiological saline flushing after3days did not see Wistar ratshad brain edema and brain damage,0.025%povidone–iodine,0.05%povidone–iodinerinse were found in3days Wistar rats had brain edema,0.025%diluted povidone–iodine set of Wistar rat edema is limited, the surrounding brain tissue structure,0.025%povidone–iodine and0.05%povidone–iodine rinse the brain tissue of rats3days laterfound positive expression of higher of NF-κB and AQP4than saline group,0.025%povidone–iodine NF-κB and AQP4positive expression with physiological saline grouphas no statistical significance (P>0.05);0.05%povidone–iodine NF-κB and AQP4positive expression with physiological saline group was statistically significant (P <0.05);0.025%povidone–iodine,0.05%povidone–iodine group rats after3days were found inserum NSE and MMP-9content were higher than in the saline group;0.025%povidone–iodine NSE and MMP-9with physiological saline groups has no statistical significance (P>0.05);0.05%povidone–iodine NSE and MMP-9with physiologicalsaline groups was statistically significant (P <0.05).Conclusion0.025%povidone–iodine rinse5min solution on the normal Wistar ratshad mild structure damage，normal brain function less affected by0.025%povidone–iodine, long-term observation effect on brain tissue to be continued;0.05%povidone–iodine rinse the brain tissue of rats is heavier damage than0.025%povidone–iodine, thebrain edema and part of the visible brain tissue damage is obvious, long-term observationeffect on brain tissue to be continued.