| Objectives To observe the effect of Resveratrol(Res)on learning and memory andmorphological changes of hippocampal tissue of Model Rats with Alzheimer’s disease(AD), in order to provide basis for anti AD drug development and clinical treatment.Methods100male SD rats were randomized into a normal sham operation controlgroup, model group, Res high dose group, middle dose group, low dose group. AD ratmodels were established by bilateral hippocampus injection of Aβ.The changes inlearning and memory ability of AD rats were observed by water maze test. The changesin Hippocampal Morphology of AD rats were determined by hematoxylin-eosin staining.HE staining was used to change the number of neurons in hippocampal CA1-CA4area ofrats and nerve cell shape molding were o-bserved after treatment. The expressions of P-tau in the hippocam-pus were meas-ured by immunohistochemical assay. Determinationof cholinesterase and the cont-ent of Apo-E4in hippocampus ELISA method. All dataswere analysed by SPSS-17.0software.Results1Compared with the sham operation group, model group rats swimming timeand the error number increased significantly (P＜0.01), compared with the model group,Res high dose group and middle dose group rats swimming time and the error numberwas significantly decreased (P＜0.05).2The nerve cells in hippocampus of coloring inrats of the sham operation group even as pale blue purple, densely arranged, nuclei,nucleoli stained; hippocampus nerve cell layers and the number of rats in model groupdecreased, the intercellular space become large, disorganized, part of the cell size, cellshrinkage, and rupture, the entire cell deep dyed red; high, medium dose group of nervecells arranged more closely; color uniformity, low dose group of neurons cytoplasmhyperchromatic, than in the model group.3Compared with the sham operation group,model group rats hippocampus P-tau integral optical density (IOD) increasedsignificantly (P＜0.01); compared with the model group, high dose group, Res in thehippocampus of rats in the P-tau integral optical density decreased significantly P＜0.05).4Compared with the sham operation group, choline ester enzymatic activity in braintissue of the rats in the model group increased significantly (P＜0.01); compared with themodel group, Res high, middle dose group rat brain tissues choline ester enzymatic activity was significantly decreased (P＜0.01), Res low dose group of cholinesteraseactivity decreased significantly (P＜0.05).5Compared with the sham operation group,the APo-E4content of hippocampus in rats of model group was significantly increased;Res high, middle dose group compared with the model group rats hippocampus APo-E4content decreased significantly (P＜0.01), Res low dose group compared with modelgroup, APo-E4content decreased significantly (P＜0.05).Conclusions1Res could improve the learning and memory ability and reduce the lossof hippocampal neurons of AD Rats.2Effect mechanism of Res may be influenced byacetylcholine metabolism, decrease the expression of P-tau, and reduce the contents ofAPo-E4function. |
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