Effects Of Starvation Following By Refeeding On Cerebral Injury In Rats

Objectives To evaluate the effects of starvation following by refeeding on braindamage by examination of the changes of s100B protein and NSE in serum, and onlearning and memory function by water maze test.Methods Eighty adult healthy male Sprague Dawley rats were randomly divided into10groups,8rats in each, including starvation groups with normal control group withnormal diet （group A-Ⅰ） and4subgroups according to starvation lasting periods of3,5,7and9day after starvation （group A-Ⅱ，A-Ⅲ，A-Ⅳ and A-Ⅴ）; andrefeeding groups with normal control group with normal diet （group B-Ⅰ） and4subgroups according to starvation lasting periods of3,5,7and9days following7daysrefeeding （group B-Ⅱ、B-Ⅲ、B-Ⅳ and B-Ⅴ）Blood samples in the abdominalaorta were gathered from group A-Ⅰ, A-Ⅱ，A-Ⅲ，A-Ⅳand A-Ⅴ for testing thecontents of serum S100B protein and NSE. Water maze tests were made in group B-Ⅰ,B-Ⅱ, B-Ⅲ, B-Ⅳ and B-Ⅴ.Results1The serum S100B protein and NSE contents were decreased in group A-Ⅱand group A-Ⅲ, with significant difference in group A-Ⅲ from group A-Ⅰ（P＜0.05）.However there were increasing trends of the serum S100B protein and NSE contents ingroup A-Ⅳ and A-Ⅴ, with significant difference in group A-Ⅴ from group A （P＜0.05）.2The water maze test results: the place navigation test: the first and seconddays of incubation periods were decreased in group B-Ⅱ and B-Ⅲ;but were increased ingroup B-Ⅳ and B-Ⅴ, with significant differences from group B-Ⅰ（P＜0.05） expectgroup B-Ⅳ（P＞0.05）; the spatial search test: there were increased on the times ofplatform quadrant in group B-Ⅱ and B-Ⅲ with no significant differences from that ofgroup B-Ⅰ（P＞0.05）; there were decrease of position residence times were shorted ingrou B-Ⅳ and B-Ⅴ, with significant difference in group J from those in group B-Ⅰ（P＜0.05）.Conclusions1Brain damage may occur when starvation last longer than7days,2refeeding following9-day starvation may also affect learning and memory function inrats.