The Biological Evaluation To The Combination After The Leaching Liquor With The Brushite Coating Dissolved Into The Different Concentrations Of Strontium

Objectives Through the detection of in vitro cytotoxicity, proliferation test and animaltoxicity test and related gene expression, to make a preliminary evaluation of the biologicalbehavior of brushite coating extracts in different concentration of dissolved strontiumcombined application, provides the possibility for the experiment and clinical development.Methods This experiment using electrochemical deposition on pure titanium surfacemodification, the surface preparation of brushite coating, surface morphology of coatingspecimens with scanning electron microscopy, and prepared into leaching solution, bymentioning the SR added different concentration solution to soak, grouping and playtogether. Incorporating the brushite samples with different content of strontiumconcentration of leaching solution directly on the MC3T3-E1mouse embryonic osteoblasts,by using the method of CCK-8, cytotoxicity and proliferation of different concentrationgroups. The inverted phase contrast microscope, observation of each concentration groupunder the growth morphology of osteoblasts, and comprehensive studies to determine theexperimental groups; the SD rats injected with brushite samples with different content ofstrontium concentration of the extract, acute toxicity in vitro stimulation test, pyrogen testand skin test, observation of injection immediately,24hours,48hours,72hours in theexperimental group and the control group rats breathing, body temperature, there is noreaction and changes of body mass poisoning, rats were injected with little parts of the skinwithout edema and erythema, with a preliminary evaluation of biocompatibility; byGiemsa staining and quantitative real-time PCR technique, observation and expression thegrowth morphology of bone cells and related gene into different concentration packetdetection.Take SPSS17.0to analysis the data, all data are Mean and Standard Deviation.Take factor analysis of variance to compare the data between the groups in the cytotoxicitytest and PCR. Take analysis of variance for repeated measurement data between the groupsin the animal toxicity test. If P<0.05, we think it be of statistical significance.Results1By changing the brushite coating sample extraction content of Strontium inproduced liquid, different strontium concentration group, cell toxicity grading was≤2,shows the strontium incorporation did not produce significant cytotoxicity, determine theexperimental group for pure titanium leaching solution group, brushite coating leaching extract group, the content of strontium is1%,5%,10%respectively the brushite coatingleaching solution group.2Acute toxicity test, pyrogen test and skin irritation test resultsshow, the experimental group and the control group in the experiment of the reaction islargely the same, have no obvious difference, that the materials of strontium doped extractshad no obvious toxicity on rats, no heat, no irritation effect. To preliminary evaluationmode of action combined with the biological materials and strontium element is safe andnontoxic to rat, has good biological compatibility.3Giemsa staining results showed thatbone cells, can have more proliferation into1%groups; real time quantitative PCR resultsindicated that, in osteoblasts after3days of culture, osteocalcin (OCN) were higher thanthose of the other groups in the brushite and1%groups, and no significant difference.Osteopontin (OPN) were lower than those of other groups in the brushite and1%groups,and no significant difference;5days later, in the OCN doped strontium concentration of1%groups of osteoblasts was significantly higher than the other expression of any group,and no significant difference between the other groups. In the OPN doped strontiumconcentration of5%group and10%group of osteoblasts was significantly higher than thatof the rest of the expression level of group, brushite group and the1%groups had nosignificant difference.Conclusions Interaction of electrodeposition of brushite coating sample extract dissolvedin1%,5%,10%strontium,1%groups of the model under the action of brushite biologicalmaterials with good biocompatibility, can promote the proliferation of osteoblast.