| Objectives To study the time-and temperature-dependent survival of ovarian oocytescollected from postmortem carcass, interval on ovarian oocyte survival.Methods8weeks old female ICR mouse were randomly allotted to group which5mouse in each group. ICR mice were killed and placed for different periods (0,2,4,6,8and10h) at room temperature (25℃), low temperature (4℃) and high temperature (37℃).After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocytemeiotic maturation rate, mitochondrial distribution and intracellular glutathione (GSH)level were evaluated.Results1. At room temperature (25℃) mice carcass could hold up to4hours and collectGV oocytes, and at low temperature (4℃) the carcass could hold up to10houres but1hours could be hold at high temperature (37℃).2. The number of collected GV oocytes inthe ovary under different conditions was counted; the result showed that it deceased as thepreservation time lasted at the same temperature.3. At the same point in time, the ratio ofgerminal vesicle breakdown (GVBD) and the first polar body emission (PBE) of oocytematuration in vitro gradually reduced as preservation temperature increased.4. The rate ofabnormal mitochondrial distribution in the preserved oocytes was obviously higher thanthat in the control oocytes, while GSH level was not altered in collected oocytes.5. Neitherchromosome arrangement nor spindle organization in experimental group was consistentwith control group.Conclusions1. The number of GV oocytes reduced after mouse carcass preservation.2.Low preservation temperature can extend the storage time of the carcass, but hightemperature can reduce the storage time.3. The preservation time affected oocytematuration in vitro and delayed oocyte maturation time.4. The rate of normalmitochondrial distribution increased after preservation.5. Chromosome arrangement,spindle organization and GSH level were not affected by preservation within a certain timeframe. |
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