Association Between MicroRNA In Plasma And Coronary Artery Disease

Objective To investigate the expression levels and clinical implication ofperipheral plasma miR-126、miR-130a、miR-222in patients with coronaryartery disease(CAD). Methods A case control study was conducted.87patients with CAD and28controls without CAD were included. Peripheralplasma miR-126、miR-130a、miR-222expression levels were detected withquantitative real-time polymerase chain reaction(qRT-PCR) analysis. Therelationship between plasma miR-126、miR-130a、miR-222expression levels inCAD patients of non-statin therapy group and the severity of coronary arterystenosis were analyzed. The differences of plasma miR-126、 miR-222expression levels between the CAD patients of non-statin therapy group andstatin therapy group were analyzed also. Results (1) Compared with controlgroup，the expression level of plasma miR-126was significantly decreased（fold0.048，P＜0.05） in CAD of non-statin therapy group and miR-222wassignificantly increased(fold4.618，P＜0.05）in CAD of non-statin therapygroup；miR-130a was decreased(fold0.210，P＞0.05）in CAD of non-statintherapy group.(2) In CAD patients of non-statin therapy subgroup，scored by theimproved Gensini method，the expression level of plasma miR-126and miR-222were significantly different in mild group and severe group. Compared withcontrol group，the expression level of miR-126was lower in severe group(mildgroup with fold0.101，severe group with fold0.027)（P＜0.05）and miR-222was higher (mild group with fold2.767，severe group with fold7.582)（P＜0.05）.(3) The expression level of plasma miR-126was lower in CAD patientsof non-statin therapy group than in statin therapy group (non-statin group withfold0.048，statin group with fold0.328)（P＜0.05） and the expression level ofplasma miR-222was higher (non-statin group with fold4.618，statin group with fold1.391)（P＜0.05）. Conclusion The expression levels of peripheralplasma miR-126and miR-222in CAD patients were closely related to theseverity of coronary artery stenosis. The expression levels of peripheral plasmamiR-126was increased and miR-222was decreased in CAD of statin therapygroup.