Pharmacokinetics Study Of Dragon’s Blood In Rats Under Simulated Weightlessness

With the development of manned space flight, health problems of the astronauts in thespace attracte widespread attention and aerospace medicine is emerged. Using rational drug inthe space environment is the focus of the content of aerospace medical research. Drugabsorption, distribution, metabolism and excretion are affected by weightless environment.onset time, duration of action, therapeutic effects and side effects are affected by drugmetabolism. Explore pharmacokinetic variation of aerospace medicine is a serious problem inaerospace medicine. Drageny I is one of rare traditional Chinese medicines, which wasresearched for many years in our group. Drageny I may play a role in aerospace medicineaccording to TCM theory. Previous studies show that Drageny I has a good protective role inthe rats’ cardiovascular system and brain damage in the weightless environment system.Therefore, this study established a model of simulated weightlessness by rats tail hanging.An HPLC-MS method for the quantification of loureirin A(LA), loureirin B(LB),loureirin C(LC), pterostilbene(PTS) and7,4’-dihydroxyflavone(DHF) in rat plasma,urine, feces, and bile was developed after oral administration of Drageny I. And anHPLC-MS method for the quantification of resveratrol(RES) in rat feces.An HPLC-MS/MS method for the quantification of LA, LB, LC, PTS and DHFin rat plasma, urine, feces, and bile was developed for the first time. Thepharmacokinetic parameters between normal and simulated weightless groupshowed that is parameters influenced by the chemical structure. Compared with thenormal group, AUC(0-T)of flavonoids is lower in simulated weightless group. AUC(0-T)of LA is30.5±2.4μg/L·h in normal but18.1±5.4μg/L·h in simulated weightlessgroup. AUC(0-T)of diphenylethene in simulated weightless group is higher than it innormal. AUC(0-T)of PTS is447±78.6μg/L·h in normal but548±92.4μg/L·h insimulated weightless group. Tmax in simulated weightless group is later than it innormal. Compared with the normal group, there are two peaks in simulatedweightless group for dihydrochalcones. It showed that the absorption rate anddisposition of Drageny I were changed in simulated microgravity.Compared with the normal group, cumulative excretion amount of compound in rat urine in simulated weightless group is higher, after oral administration ofDrageny I. The cumulative excretion amount of LA in simulated weightless groupis5times of it in normal. The cumulative excretion of compounds in simulatedweightless group were much higher than it in normal in bile.The cumulativeexcretion amount of diphenylethenes in rat feces in simulated weightless group ismuch higher than it in normal.But the cumulative excretion amount of flavone in ratfeces is opposite.