Effects Of BMSC Transfected With ADAM17-shRNA On The Human MCF-7Breast Cancer

Objectives To study the effects on the proliferation and invasion ability of MCF-7breast cancer, which co-culture with rat bone marrow mesenchymal stem cell transfectedwith ADAM17-shRNA. To explore new ways to transfer ADAM17-shRNA, for targetedtherapy of breast cancer by targeting ADAM17provide a new method.Methods4recombinant lentiviral vector shRNAs were designed for ADAM17,which were transfected into MCF-7human breast cancer cells. The expression ofADAM17mRNA in MCF-7were detected by real-time PCR to screen out the best shRNA,which the interference on the expression of ADAM17. The BMSC from SD rat werecultured, the BMSC transfected with ADAM17-shRNA were co-cultured with MCF-7, theexpression of ADAM17mRNA in co-cultured cells were detected by real-time PCR, theinvasive ability of MCF-7cells was studied by Transwell method, and the proliferativeability of MCF-7were detected by MTT assay.Results The shRNA-has-1658, which selected by real-time PCR, can inhibit MCF-7cell line ADAM17mRNA. Real-time PCR were used to detect the expression levels ofADAM17after co-cultured, the control group of the ADAM17mRNA relative expressionlevel is set to100%. The results showed that, ADAM17-shRNA can obviously inhibit theexpression of ADAM17mRNA, comparing with the control group, the difference wassignificant. The number of MCF-7breast cancer cells through the membrane in nonsensegroup and control group were more than ADAM17-shRNA group, ADAM17-shRNAgroup compared with the control group was significant difference. The MTT resultsshowed that, the proliferation of MCF-7on ADAM17-shRNA group was inhibited,compared with the control group was significant difference.Conclusions ADAM17mRNA expressions was significantly decreased, and theproliferation and invasive ability of MCF-7cells was markedly inhibited by BMSC-ADAM17-shRNA.It suggests that BMSC as the carrier of the ADAM17-shRNA can beused for targeted therapy of breast cancer.