| Objective1Observe the immune function of1,25-dihydroxy Vitamin D3towards mice adjuvantarthritis (adjuvant arthritis, AA), and investigate the regulatory effects and the possiblemechanism of1,25(OH)2D3on immunity mediated by T lymphocyte.2Establish the mice penetrating keratoplasty model, and investigate inhibitory effectand its possible mechanism of1,25-dihydroxy Vitamin D3on corneal allograftimmune rejection.Method1C57BL/6mice were randomly divided into3groups：normal group, AA group andintervention group，6mice in each group. Except the normal group,AA model wasinduced by subcutaneous injection of complete Freund’s adjuvant in the right plantar ofeach mouse, then strengthen the immune response via injecting the same dose ofcomplete Freund’s adjuvant one week after the first injection. Since the day after the twoinjection, the intervention group received intraperitoneal injection of1,25(OH)2D3,1time per day,100ng per time, while t normal group and AA group groups received thesame dose of saline. According to this method, alternate day dosing once, for twoconsecutive weeks. Then observe the effect of1,25(OH)2D3on spleen index, thymusindex and peripheral blood T cell subsets.2C57BL/6mice were used as receptors and Bab/c mice as donors to establish thecorneal penetrating transplantation model. C57BL/6mice were randomly divided into3groups：normal group, transplantation group and intervention group，6mice in eachgroup. Except the normal group, the right eyes of the other two groups underwentallogeneic corneal penetrating keratoplasty combined with skin transplantation. Thenext day after surgery, the intervention group received intraperitoneal injection of1,25(OH)2D3, one times per day,100ng per time, while the rest groups received the samedose of saline. According to this method, alternate day dosing time continuous13days.On day13after the operation， analyse the CD4+/CD8+T lymphocyte ratio and γδT cell ratio of the peripheral blood，detect the expression of IFN γ mRNA, IL-10mRNAand IL-17mRNA of spleen lymphocyte. Observe the corneal graft and skin graftsurvival situation until13days.Result1On day21，the spleen and thymus gland index in intervention group was significantlylower than AA group. When compared with AA group, the number of CD4+cells inintervention group decreased obviously, the number of CD8+cells increase significantly,the ratio of CD4+/CD8+decreased significantly. The difference was statisticallysignificant.2The turbidity、 edema and neovascularization of the corneal graft in interventiongroup was lower than the transplantation;The survival period of skin graft in the groupintervened by1,25dihydroxyvitamin D3was longer than the transplantation group;Onday13after the operation, The ratio of CD4+/CD8+in intervention group andtransplantation group were （2.36±0.41）and （3.19±0.53）on day13post-operation,P <0.05, the difference was significant; Compared with the normal group, the IFN-γmRNA and IL-17mRNA expression of transplantation group increasedsignificantly,IL-10mRNA increased significantly; when compared withtransplantation group, the IFN-γ and IL-17mRNA expression level of1,25-dihydroxyVitamin D3group decreased significantly. γδT cells of peripheral blood intransplantation group was significantly higher than the normal group, however， theintervention group decreased obviously.Conclusion11,25(OH)2D3can improve the immune function disorder of AA model mice byregulating the cell number and proportion of CD4+, CD8+subsets.21,25(OH)2D3might increase the number of CD8+T cells, decrease the number ofCD4+T cells and the CD4+/CD8+ratio of the peripheral blood of the penetratingkeratoplasty group.1,25(OH)2D3inhibited the immune rejection by adjusting the CD4+and CD8+T lymphocyte subsets.31,25(OH)2D3might down-regulate the ratio of γδT cells cells which boost theinflammatory effect, then inhibit the immune rejection of penetrating keratoplastygroup.41,25(OH)2D3inhibited the expression of Th1related IFN-γ mRNA and Th17related IL-17mRNA， and significantly improve the expression of Th2relatedIL-10mRNA. |
PDF Full Text Request