| Object In recent years the experiment was vascular transplantation in the treatment of lower extremity arterial occlusive disease more widely applied, but after surgery graft restenosis constraints in this operation. Restenosis after transplantation study found that the main mechanism is vascular graft intimal hyperplasia lead to vascular occlusion, endometrial hyperplasia vascular inflammation and the formation, endometrial leukocyte adhesion aggregation, migration and proliferation of smooth muscle cells and extracellular matrix accumulation pathological process, and the Adhesion molecule is above all important in the pathogenesis of the foundation. This study through the control of the vein graft experimental animal model study on rats autologous vein graft intimal hyperplasia extent and impact of transplantation of animal CD11b/CD18adhesion factor expression, for further treatment based on clinical. Methods To establish an animal model will be divided into three groups of laboratory animals, the experimental group A (n=24) intraperitoneal injection CD11b/CD18the monoclonal antibody0.1mg/d continuous injection of28days, experimental group B (n=24) intraperitoneal injection of IL-60.1mg/d injection for28days, the control group, Group C (n=36) did not use any drug intervention. l0g/L pentobarbital sodium40mg/kg intraperitoneal injection of anesthesia, after the success of anesthesia, the incision from the center of the neck, the anatomy of rats exposed external jugular vein, cut by4mm vein of vascular transplant, with intravenous heparin lumen physiological Drip irrigation. Ipsilateral carotid artery at both ends of free and blocked, which cut off. Will be home between the vein graft transplant in the carotid artery,11-0no damage done end-to-end anastomosis suture, a blood test to confirm graft blood vessels open after the incision closed. Experimental groups A, B, respectively7,14,21,28days were cut, C1,3,7,14,21,28days in the group were admitted. Anesthesia success, for cervical midline incision, the anatomy of exposure and free Vein graft, cut vein graft, placed in frozen section of5μ m consecutive slices, four percent more POM fixed and placed in-70℃preservation, vein graft specimens, HE staining, observed under an optical microscope blood transplant pipe wall thickness, the lumen Size and intimal hyperplasia situation. Image analysis system were measured endometrial thickness (IT), in the film thickness (MT), endometrial area (IA), in the film area (MA), lumen area (LA), calculated IT/MT, IA/MA ratio. Blood samples taken from rats1ml, a blood anticoagulant heparin, on the flow cytometry (EPICS2XL Coulter of the U.S. company’s products) detection, CD11b/CD18number of elements, each sample of5,000cells, results in an average of fluorescence intensity (MFI, a few units for the Road). Immunohistochemisis software and computing artery in the film endometrial endothelial cells, macrophages and smooth muscle cells were stained markers. Results CD11b/CD18flow cytometry analysis showed that the expression: A, B, C group CD11b/CD18changes in the expression of two weeks after transplantation, gradually increased over time, about two weeks has increased significantly, after gradually stable,3thoughtful four weeks after the rate of increase gradually decreasing steadily, the group of blood samples found in the flow cytometry measurement:A group with the C group, A group CD11b/CD18expression of a significant reduction (P<0.05); B group and the group C In comparison,CD11b/CD18expression levels increased significantly, a different treatment, a significant difference (P<0.05) vein graft pathological analysis:vein graft histological sections, HE staining, observed under an optical microscope, SD large Jugular normal mice have a thin membrane, are2-3in the film of smooth muscle cells, a small interval of a small amount of collagen and elastic fibers. Thick outer membrane, formed by the collagen fibers. With the normal jugular vein, compared to transplant three days after the vessel began to endometrial hyperplasia, began to appear one week after endometrial hyperplasia, light microscope can be observed8-12of the smooth muscle cells, the cells are disordered, A and Group Group B after one week also found that endometrial changes, A group proliferation of smooth muscle cells rise significantly less than in group C, while Group B smooth muscle cell proliferation significantly more than the rise in group C, leading to significantly narrow the lumen vein graft. After Group C and Group A, B and C of the IT/MT, IA/MA of the differences were significant (P <0.05). Immunohistochemical staining showed that the DAB:CDllb/CD18expression in the cell cytoplasm Or membrane of brown granular precipitation, three rats were seen within the vein graft is positive. Each group with the passage of time and yellow particles increased, reached the peak within two weeks, and then CD11b/CD18of stabilizing. CD11b/CD18monoclonal antibody group in the test group endometrial endothelial cells, macrophages and smooth muscle cells only see scattered brown granular positive staining substances, the control group and endometrial thickening of the arteries in the membrane of the endothelial cells, macrophages And smooth muscle cells seen in a lot of brown granular CD11/bCD18positive staining substances, part of integration into tablets, CD11b/CD18monoclonal antibody group CD11b/CD18positive staining material was significantly lower than injection of IL-6, of the three groups more Significant difference (P<0.05).Conclusion his study showed that rats autologous vein graft intimal hyperplasia after CDllb/CD18extent and the expression of a positive correlation, anti-CD11b/CD18monoclonal antibody CD11b/CD18can reduce the expression and inhibition of neointimal hyperplasia, and white-inflammatory cytokines-To increase the expression of CD11b/CD18, and to promote endometrial hyperplasia. So in rat CD11b/CD18can inhibit the expression of the vein graft neointimal hyperplasia, has important clinical significance. |
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