Experimental Research Of Compatibility Of Mulberry Leaf And Chrysanthemum In The Mechanism Of Fever

Objective:By the investigation of the effect on lowing body-temperature, anti-inflammation, and fighting free radicals injury of compatibility of mulberry leaf and chrysanthemum in fever rats to demonstrate that the compatibility of the two drugs can enhance the above functions and find out the best compatible proportions of the two medicines on each aspects. Methods:sixty-four SD half male and half female rats are randomly dividing into8groups:blank group, model group, aspirin group, mulberry leaf group, chrysanthemum group, mulberry leaf and chrysanthemum5:2group, mulberry leaf and chrysanthemum2:1group, mulberry leaf and chrysanthemum1:1group;8rats each. Besides control group, other rats are induced by the injection of15%dry yeast suspension10ml/kg in the back skin to establish the fever rat models. And then take the mulberry leaf, chrysanthemum, mulberry leaf and chrysanthemum compatible proportions of5:2,2:1and1:1into the physic liquor, make the intragastric administration as the treatment respectively. Observing body-temperature, the content of IL-1β, TNF-α, MPO and NO, the activity of iNOS in blood serum of the fever rats. Results:(1) mulberry leaf group, chrysanthemum group and their different compatible proportions all have the effect of lowing the temperature, and compared to model group (P<0.05or P<0.01), the compatible proportion of1:1group has the best effect in this aspect.(2) mulberry leaf, chrysanthemum and their different compatible proportions all have the effect of decreasing the content of IL-1β, TNF-α, MPO, NO and iNOS in blood serum, and compared to model group (P<0.05or P<0.01), the combined ratio of1:1has the best effect in this aspect. Conclusion:(1) Compatible application of mulberry leaf and chrysanthemum has significantly synergistic effect on lowing the temperature, anti-inflammation, and fighting free radical injury, which reveals the scientific character of compatible application.(2) The best compatible proportion on lowing the body temperature, anti-inflammation, and fighting free radical injury is1:1.(3) The mechanism of bringing down the fever of compatible application of mulberry leaf and chrysanthemum might be through decreasing the content of IL-1β, TNF-α, MPO in blood serum to lowing the body-temperature indirectly; by reducing the content of NO and iNOS and other free radicals in blood serum to reduce body damage from heat.