Research On The Relationship Between The TaqI B Polymorphism Of The Cholesterol Ester Transfer Protein Gene And Atrial Fibrillation In The Han And Kazak Populations

Objective:Atrial fibrillation(AF) is one of the most common and complex clinical arrhythmia. In recent years, studies on CETP Gene polymorphism and myocardial fibrosis in AF have acquired attention. The relationship between the CETP gene TaqlB and AF in Kazak and Han population of Xinjiang is discussed in this paper in order to further clarify molecular mechanisms of atrial fibrillation. Methods:This research, using case-control design, was carried out on103Kazakh and101Han patients with ECG-evidenced AF, case group, and101Kazakh and129Han subjects without ECG-evidenced AF, control group. DNA was extracted,TaqIB polymorphism of CETP was detected by polymerase chain reaction(PCR), and CETP Gene TaqIB polymorphism of Chymase gene was detected using PCR restriction fragment length polymorphism (RFLP).Distributions of different genotypes and alleles of four groups were compared, using SPSS version17.0The Hardy-Weinberg equilibrium was assessed by chi-square analyses, intergroup differences in frequency of alleles and genotypes analysed using x2test, and differences were found statistically significant when P<0.05. Results:We analyzed intergroup differences of different genotypes, different loci, biochemical parameters and clinical data, and found:(1)Homozygous deletion (B1B1type), heterozygous (B1B2type) and insert homozygotes (B2B2type) genotype of CETP satisfied Hardy-Weinberg distribution.(2) Comparison of B1B1polymorphism and B1allele frequency between case and control groups of Han and Kazakh subjects were found statistically significant(P<0.05). Frequency of B1B1genotype of CETP gene in the case group was significantly higher than in the control group in Han population(34.7%and26.4%,respectively x2=10.686, P=0.001).(3)Frequency of B1B1genotype of CETP gene in the case group was significantly higher than in the control group in Kazak population(53.4%and24.8%respectively, X1=27.802,P＜0.001).(4) Logistic regression analysis revealed B1B1polymorphism of CETP TaqIB loci is significantly different between case and control groups of Han and Kazak population. Conclusion:Polymorphisms of CETP TaqIB might be related to non-valvular AF among Han and Kazak population. Significant difference of inter-ethnicity exists CETP Taq IB polymorphism between AF patients of Han and Kazak population. CETP Taq B1allele is a possible independent risk factor for genetic susceptibility to AF.