| Objective:Through quantitative and qualitative analysis the active ingredients established the quality standards of Song bu li oral liquid. To provide a stable and reliable quality control method for medicinal quality of Song bu li oral liquid,provi de research data for the development and application.Methods:According to"Chinese Pharmacopoeia"in the mixture of quality requirements, the Song bu li oral liquid oral relative density,Ph,heavy metal content checks,and using thin layer chromatogra Phy on loose fill power oral nard new ferulic acid and ketone qualitative identifica tion; using content visible spectro Photometry loose fill power oral total polyPhenols and total polysaccharides, total polyPhenols detection wavelength was760nm,the total polysaccharide detection wavelength was610nm.HPLC method for loose fill power oral ferulic acid, ketones nard new determination.Column:Hypersil ODSCig (250mm×4.6mm,5μm); mobile Phase:methanol:1%acetic acid in water(seventy-eig ht past ten p.m.),methanol:water (60:40),flow ratel mL/min,detection wavelength wa s320nm,250nm,column temperature was35℃. Results:Song bu li oral liquid rel ative density of not less thanl.02,PH value of between3to5,heavy metal meets US P standards. Gallic acid and glucose were used as loose fill power oral total polyP henols and total polysaccharides of reference,within the range of concentrations of1.826-6.662μg/mL(r=0.9990)and0.015-0.105mg/mL(r=0.9994)There was a good lin ear relationship. Ferulic acid in the20μg/mL-140μg/mL range,the sample volume and the peak area of ferulic acid has a good linear relationship(r=0.9966);average r ecoveries were98.13%,RSD was0.989%.Nardosinone within0.02-0.08μg/mL range nard new ketone injection volume and peak area has a good linear relationship,(r=0.9994);average recoveries100.76%,RSD wasl.24%.Conclusion:Established qualitati ve and quantitative detection method is simple,accurate,reproducible,and can be used to effectively control the Song bu li oral liquid quality. |
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