The Detection And Identification Of Brucella From Human Infection And MLVA Genotyped Research

Objective:To identify and genotype by multiple loci variable number tandem repeat analysis (MLVA) for Brucella collecting from Xinjiang region. Method:62suspected positive patients infected with Brucella were collected by complains, sign, history of pathogen exposure, etc. After Rose-Bengal plate agglutination test for first screening, blood cultures were performed to isolate the Brucella. Then gram stain, catalase test, oxidase test and Vitck2compact were used to identify the Brucellas. PCR and MLVA were performed to genotype Brucellas.16sites of each Brucellas were analyzed. Result: Of62suspected Brucella positive patients,34were positive for Rose-Bengal plate agglutination test and12were identified as Brucella melitensis, which all infected sheep and goats. The results of genotyping by MLVA-8indicated that7were type45,1was type42,1was type62and3were unknown types. Genotyping by MLVA-Hand16had no results conforming to known genotypes in Genbank. Conclusion:MLVA-16could use to genotype Brucella and Panel2had high resolution. Brucella was mainly infected sheep and goats in Xinjiang region. The genotyping results of12Brucella were different to known sequences in Genbank.3Brucellas identified by MLVA-8were new genotypes, showing as (1-5-3-12-2-3-3-2) and (1-5-3-11-2-3-3-2) respectively.