Research On The Mechanism For Myocardial Cells Apoptosis In Histone Acetylation After Heavy Training By Moxibustion

Objective:This study is to find out the endogenous mechanism of histoneacetylase p300、CBP by moxibustion in myocardial apoptosis after heavy exercise, and will be used to protect the heart during heavy sports training, by observing the apoptosis-related factors Fas, Bcl-2and p300、CBP histone deacetylase apoptosis relationship to explore p300、CBP histone deacetylase anti-apoptotic role in the myocardium moxibustion, as in sports training to effectively protect the heart provides a new research ideas.Methods:60adult KM mice were randomly divided into blank control group (A), exercise control group (B), exercise and moxibustion therapy group (C)(n=20).Both group B and group C kept swimming training every day, while group A was free activity during the time.In the first3days, let the mice swim for30minutes a day adaptation.24h after the last swimming training,the three groups of mice were killed off the vertebral quickly take heart tissue, paraffin embedded, sliced for immunohistochemical detection of Fas, Bcl-2, p300, CBP expression,, and cardiomyocyte apoptosis detection.Results:(1)Fas protein expression:B Compared with A, the Fas protein expression was significantly increased (P<0.05); C compared with B, the Fas protein expression was significantly decreased (P<0.05), C Compared with A, the result is insignificant(P>0.05).(2). Bcl-2protein expression:B Compared with the A, the Bcl-2protein expression was significantly decreased (P<0.05); Bcl-2protein expression increased significantly when C compared with B (P<0.05).(3)p300expression:A Compared with C, p300expression was significantly decreased (P<0.01); p300expression of C was significantly higher than B (P<0.05).(4) CBP expression:B Compared with A, CBP expression was significantly decreased (P<0.05); C was significantly higher than B (P<0.05).(5) Apoptosis Detection:B Compared with A, apoptosis rate was significantly increased (P<0.01);there is significance between A and C(P<0.05); apoptosis rate of C was significantly decreased than B (P<0.05).Conclusion:Moxibustion can promote the expression of p300、CBP in mouse myocardial cells after heavy training, thereby inhibiting the apoptotic process started, so that decreased expression of death receptor Fas, increased expression of Bcl-2,so that to decreased the myocardial cells apoptosis rate after training and protect heart function.