Serum HBsAg And HBV DNA Quantitative Level In A Preliminary Study On The Significance Of HBV Related Liver Failure

Objective The aim of this study is to analyze the quantitativedetection of serum HBsAg and HBV DNA level in the significance ofpatients with hepatitis B virus (HBV)-related liver failure.Methods Choosing97cases of patients with chronic liver failure(CLF),108cases of patients with acute-on-chronic liver failure (ACLF)and123cases of patients with chronic hepatitis B viral (CHB).Electrochemical luminescence method to detect serum HBsAg and HBeAg,fluorescence quantitative PCR method to detect serum HBVDNA level.Three groups were compared between patients with HBsAg and HBVDNA levels, three groups of different HBeAg status and HBsAg levels ofHBV DNA, and the related biochemical indexes of correlation.Results The serum HBeAg negative rate in the CLF group, ACLFgroup and CHB group were68.4%(67/98),42.9%(51/119),8.78%(60/123), the difference was statistically significant (P <0.05). In the threegroups of serum HBsAg level comparative difference was statisticallysignificant (P <0.01), while HBV DNA is difference with no statistical significance (P>0.05). Three groups of serum HBeAg positive patientswith HBV DNA were higher than in patients with serum HBeAg negativegroup (P <0.05). Serum level of HBsAg in HBeAg ACLF groupcomparing differences between positive and negative patients have nostatistical significance (P>0.05). Three groups of serum HBsAg levelsand HBV DNA levels were positively correlated (P <0.01), and alanineamino transaminase (ALT), aspartate amino transaminase (AST) comparethe difference was statistically significant (P <0.05), but the rest of thebiochemical index of total bilirubin, albumin, AFP, prothrombin activity,comparative differences are not significant (P>0.05); HBV DNA level inACLF difference compared with with the ALT, AST and CHB groups wasstatistically significant (P <0.01). Two different hepatic failure groupserum HBsAg levels in HBV DNA levels, ALT and AST meaningfulcomparison difference (P <0.05)； total bilirubin, cholinesterase, albumin,prothrombin activity, a tire protein, blood ammonia is meaningless (P>0.05).Conclusion The level of the serum HBsAg in the acute-on-chronicliver failure and chronic liver failure parallel to the severity of the diseasewithout direct relation.