The Expression And Significance Of TGR5and NF-κB In The Placenta Tissue Of Gestational Diabetes Mellitus

Objective：To study the inflammatory expression and level of TGR5and NF-κB in the placenta of full-term gestational diabetes mellitus andnormal pregnant women, and to explore the relationship between theinflammatory pathogenesis and changes of expression of TGR5and NF-κB.Methods：From May to November2013, forty placenta were collectedfrom the first Affiliated Hospital of Chongqing Medical University,including twenty with GDM and twenty with normal pregnancy. Weperformed immunohistochemical test to detect the expression and locationof TGR5and NF-κB in the placenta and made semi-quantitative analysis.At the same time, sixty serum collected including twenty with type2diabetes mellitus, twenty with GDM and twenty with normal pregnancy todetect the quantity of TNF-α by the means of double antibody sandwichELISA test.Results：(1) The level of TNF-αin the serum of type2diabetes mellitus (495.0640±15.11716) was significantly higher than that inpreoperative GDM group (18.8718±1.63995) and preoperative normalpregnancy group (7.8304±1.39589)(P<0.05). The level of TNF-αin theserum of preoperative GDM group (18.8718±1.63995) was significantlyhigher than that in preoperative normal pregnancy group (7.8304±1.39589)(P<0.05). The level of TNF-α in the serum of preoperative normalpregnancy group (7.8304±1.39589) was significantly higher than that inpostoperative normal pregnancy group (5.9007±0.75261)(P<0.05). Thelevel of TNF-αin the serum of preoperative GDM group (18.8718±1.63995) was significantly higher than that in postoperative GDM group(9.8432±1.39530)(P<0.05).(2) There were the expression of TGR5andNF-κB in the placenta of both gestational diabetes mellitus and normalpregnancy. Both TGR5and NF-κB were mainly localizd in the cellularmembrane and cytoplasm of the macrophages and syncytiotrophoblasts.The level of TGR5expressed in the syncytiotrophoblasts of GDM groupwas significantly lower than that in the normal pregnancy group(Z=-2.105,P=0.035). The level of TGR5expressed in the macrophages ofGDM group was also significantly lower than that in the normal pregnancygroup (Z=-2.321,P=0.020). The level of NF-κ B expressed in thesyncytiotrophoblasts of GDM group was significantly higher than that inthe normal pregnancy group (Z=-2.434,P=0.015). The level of NF-κBexpressed in the macrophages of GDM group was also significantly higher than that in the normal pregnancy group (Z=-2.266,P=0.023).(3) Therewas significantly negative correlation between the expression of TGR5andNF-κB in the placental syncytiotrophoblasts from GDM group (r=-0.55，P=0.012). There was also significantly negative correlation between theexpression of TGR5and NF-κB in the placental macrophages from GDMgroup (r=-0.446，P=0.048).Conclusion：The down-regulation of TGR5and up-regulation of NF-κB may be involved in the pathogenesis of the gestational diabetesmellitus.