| Objective: To understand the role of cytokine induced killer (CIK)cells on human colon cancer cells and provide experiment basis for clinicaltreatment of colon cancer.Methods: Human peripheral blood mononuclear cells (PBMC) wereseparated from healthy people. IFN-γ, CD3monoclonal antibody (mAb)and recombinant human interleukin-2(rh IL-2) were added into the culturemedium to induced CIK cells mature. Flow Cytometry (FCM) was used todetect the phenotype of CIK cells and CD3+CD56+double positive CIKcells should be more than20%. We cultured SW480colon cancer cell lineindividually (control group) or co-cultured with CIK cells (co-culturedgroup). The levels of cell proliferation, migration and invasion were testedby cell counting kit-8(CCK-8), wound scratch and transwell invasion assay,respectively.Results:①The purity of CD3+CD56+double positive CIK cellsinduced by culture system of IFN-γ、CD3mAb and rhIL-2was31.7%. Thepurity of CD8+CD4-and CD4+CD8-cells was76.1%and17.9%,respectively.②The result of CCK-8test showed CIK cells can significantly inhibit the proliferation of SW480cells. Wound scratch assayresult showed that the average repair residual area of co-cultured group(9.36±0.79) was significantly larger than that of control group (5.78±5.78)(P <0.01). Transwell invasion assay result showed that the number ofinvasive cells of co-cultured group (113±4.57) was much smaller than thatof control group (316±9.35)(P <0.05).Conclusion:①The culture system of IFN-γ, CD3mAb and rhIL-2can effectively induce high percentage of CD3+CD56+double positiveCIK cells, and majority of those cells were CD8+cells.②CIK cells cansignificantly inhibit the levels of proliferation, migration and invasion ofSW480cells. |
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