The Effect Of Post-stroke Stress On Cognitive Function And The Expression Of Hippocampal MAP2in Rats

Aims:①To study the effect of post-stroke stress on cognitive function.②Toclear the effect of post-stroke stress on the expression of hippocampal microtubuleassociated protein2(MAP2) in rats.Methods:①The focal cerebral ischemic rat model is set up by using the middlecerebral artery occlusion (MCAO) surgery.②The depression treatment is handled byusing chronic unpredictable mild stress (CUMS) for3consecutive weeks.③Thecognitive function of the PSD rats is assessed by the Y-maze test and novel objectrecognition (NOR) tests.④The MAP2expression of hippocampus is observed by thewestern blot (WB).Results:①Ischemic brain injury or chronic unpredictable stress could decreasesignificantly the rat weight, sucrose preference, the horizontal movement and verticalmotion of open filed tests compared with the sham groups.②Ischemic brain injury orchronic unpredictable stress could decrease significantly the function of learning andmemory like the Y-maze test with decreased correct alternation rates and the novelobject recognition tests with decreased discrimination index compared with thecontrol. And the combined effect of the two is more obvious.③Ischemic brain injuryor chronic unpredictable stress could decrease significantly the expression of MAP2in the hippocampus, which shows in both MCAO group and stress group comparewith sham group, and the combined effect of the two like PSD group decreased moresignificantly.Conclusion:①Post-stroke stress aggravates cognitive function impairment ofstroke rats.②Post-stroke stress down-regulates the expression of MAP2inhippocampus.