Effects Of Cigarette Smoke On The Expression Of Cyclophilina In Rat Lung Tissueandtype Iialveolarepithelial Cells

[Objective]To research the expression of CyclophilinA(CypA)in the cigarette smoke-induced rat lungtissues;To observe the expression of CypA in AECII which exposed to cigarette smokeextract (CSE) and the relationship with ERSIA.[Methods]The entire experiment was divided into two parts:animal research and cell research in vitro.1.Animal: Forty SD ratsdivided into four groups: Control group, exposed tocigarette smoke2months,4months and stopped smoking for1month. Detecting of lungfunction in each group of rats.Extracting each groups of rats lung tissues.Using reversetranscription PCR (RT-PCR), Western blot and immunohistochemistry (IHC) method todetect the expression of CypA.2.Cell: RLE-6TN cells were cultured and treated by10%CSE for0h,3h,6h,12h,24h;Using RT-PCR, Western blot, immunofluorescence to detect the expression of CypA,Caspase12, JNK, P-JNK, CHOP.[Results]：1.Animal research:The pathology of lung tissue showed that the lung structure appears disorder exposedto cigarette smoke2months, and worse in4months. There is no significant differencesbetween exposed to cigarette smoke4months and ex-smoking rats.Western blot results showed that the expression of CypA protein in control group,exposed to CSE2months,4months, ex-smoking is0.62±0.02,1.02±0.27,1.32±0.16,0.84±0.01. The expression of CypA is highest in the rats after exposed to CSE 4month,followed by2months and ex-smoking rats,and the lowest in the controlgroup.RT-PCR and IHC results were consistent with the IHC.2.Cell research:(1)RT-PCR results showed that the expression of CypA mRNA in different time is0.63±0.23,0.86±0.01,0.88±0.09,1.11±0.09,1.02±0.01.With the extension of CSE duration,the expression of CypA presented an increasing trend, and is the highest at the12H.(2)Western blot results showed that the expression of CypA protein in different time is0.08±0.02,0.15±0.03,0.24±0.03,0.31±0.02,0.45±0.03. The protein of CypA presented anincreasing trend, and is the highest at the24H. The expression of totalJNK,P-JNK,Caspase12,CHOP protein in different time are1.52±0.22,1.13±0.24,1.50±0.21,1.21±0.55,1.04±0.24;0.16±0.05、0.29±0.03、0.38±0.03、0.48±0.06、0.69±0.01;0.12±0.05,0.25±0.04,0.39±0.02,0.58±0.05,0.49±0.02;0.00,0.35±0.09,0.48±0.04,0.66±0.06,0.81±0.08.The total JNK protein expression had no statistically significantdifferences between the control group and the CSE groups(P>0.5). P-JNK、Caspase12andCHOPexpression with the role the extension of time was a consistent increase.[Conclusion]：The expression of CypA is increased in the cigarette smoke-induced rat lung tissues, CSEcan cause CypA incresed in AECII and simultaneously activated ERSIA-related pathway,suggesting that CypA may have a protective effect in the CES induced ERS.