Experimental Study Of Sanqi On The Prevention And Treatment Of Traumatic Proliferative Vitreoretinopathy

Objective:By establishing rabbit traumatic proliferative vitreoretinopathy models, methods of using the sanqi orally, were compared with the model group was observed in each group classification rabbit model of proliferative vitreoretinopathy, and retina of basic fibroblast growth factor (bFGF) and expression of proliferating cell nuclear antigen (PCNA), and explore the prevention and treatment of rabbit traumatic proliferative vitreoretinopathy effects of sanqiMethods:The20healthy adult rabbits were randomly divided into experimental and control groups. Each group has10. Each group underwent traumatic PVR eyes modeling in the experimental group were given sanqi solution, morning fasting intragastric once (5mL/each), control group received saline, administered continuously for28days. Observed each group model eye PVR grade at the1、7、14、21、28day. The28day of air injected into the ear vein of animals in each group were sacrificed, take the eye wall of the retinal tissue, HE staining of retinal separation, and apply immunohistochemical staining was observed in bFGF and PCNA expression in the retina.Result:1,The experimental group at1day,7days appear PVR or PVR grade compared with the control group, no statistically significant (P>0.05); while in the first14days,21days,28days PVR or PVR grade appears obvious lower than the control group, there were significant (P <0.05).2,The expression of bFGF in the retina in the experimental group and the control group retinal expression of bFGF was not significantly different (P>0.05), the expression of the strength of the experimental group was significantly lower than the retina PCNA expression intensity of PCNA retina control group, there are statistics significance (P<0.05).Conclusion:1,Comparison based on hierarchical situation in the experimental group and the control group PVR prove sanqi can effectively inhibit the occurrence and development of traumatic proliferative vitreoretinopathy.2, According to the results of immunohistochemistry experimental group and control group retina bFGF and PCNA analysis shows that sanqi may inhibit PCNA expression in the retina, thus prevention of traumatic proliferative vitreoretinopathy occurrence and development.