| Objective: Adiponectin (APN) is an adipocyte-specific plasma proteinwith anti-diabetic properties, which has been recently revealed to haveanti-inflammatory activity in heart and renal ischemia-reperfusion injury.Little is known about the function of APN in bile duct ischemia-reperfusioninjury after liver transplantation. The aim of this study is to investigate therole of APN on bile duct injury in liver ischemia reperfusion injury (IRI)and clarify its possible mechanism..Methods:(1) To build a70%liver ischemia reperfusion injury modeland observe the expression of adiponectin.36male Sprague-Dawley ratswere randomly divided into three experimental groups: a sham group(n=12), a control group (n=12), and a APN group (n=12). Specimen wascollected at3,6,12and24h after operation. Alanine aminotransferase(ALT), alkaline phosphatase (ALP) and total bilirubin (TBIL) in serumwere measured. Pathological examination was assessed the bile duct injury.(2) Tumor necrosis factor-α (TNF-α) and interleukin-6(IL-6) in serumwere detected. The mRNA of TNF-α and IL-10in bile duct tissues wereassessed.(3) Apoptosis of bile duct cells was evaluated by TUNEL assay. Immunochemistry for bile duct Fas was performed. Caspase-3protein wasmeasured by western blot.Results:(1)Prolonged liver ischemia reperfusion time can cause thesevere bile duct injury; Bile duct histopathology score showed differencesbetween groups were significant, which were0,8.34±1.16,4.67±1.52,respectively. The levels of ALT, ALP and TBIL increased in control groupcompared with other two groups (P<0.05).(2) The APN group had a lowerlevels of TNF-α and IL-6than the control group(P<0.05). The level ofTNF-α decreased while IL-10mRNA increased in APN group(P<0.05).(3)It was found that adiponectin was significantly reduced apoptosis of bileduct cell in TUNEL assay. Pre-administration of APN significantly reducedthe protein of Caspase-3and Caspase-3protein expression levels were8.11±1.44,73.26±7.21,42.48±3.37, which was a significant difference (P<0.05) between the two groups. Immunohistochemical detection of Faspositive cell were3.13±1.52,56.67±9.61,32.78±9.52,which was asignificant difference (P<0.05).Conclusions:1. Liver ischemia-reperfusion injury can cause thesevere bile duct injury.2. APN protects the rat bile duct against the bileduct I-R injury after liver ischemia-reperfusion injury by a mechanism ofpro-inflammatory cytokines down-regulation, such as, IL-6and TNF-α, andanti-inflammatory cytokines up-regulation, such as IL-10.3. Adiponectintreatment may protects the rat bile duct against the bile duct I-R injury after liver ischemia-reperfusion injury by apoptosis. |
