The Expression Of MiR-449a In Colorectal Cancer And Its Significance

Objective Colorectal cancer is the third most common malignant tumors in the world,surgery is the only efficient approach to cure colorectal cancer.90%of patients can be cured if surgery is performed at the early stage,5-year survival rate of patients with advanced colorectal cancer is only about40%, and about50%of patients died of distant metastasis.Therefore, in order to improve the cure rate and prognosis of colorectal cancer,it is of great significance to further understand the pathogenic and metastatic mechanisms,and to investigate specific efficient early diagnosis indicators.MicroRNAs (miRNAs) are class of endogenous non-coding single-stranded RNAs,containing about20nucleotide molecules,they comple-mentarily bind to the3’untranslated region of the target mRNA for degradation or translational repression and regulate gene expression at the post-transcriptional level.Studies have shown that miRANs have regulatory role in formation and metastasis process of colorectal cancer and other tumors.MiR-449a is a newly emerging miRNA in cancer research, miR-449a are downregulated in prostate cancer, bladder cancer, breast cancer,ovarian cancer, lung cancer,gastric cancer,liver cancer and other tumors,which can inhibit cell growth and promote apoptosis,also associated with tumor progression.However,the expression and biological function of miR-449a in colorectal cancer remain unreal.In the present study,we investigated the expression of miR-449a in human colorectal cancer tissues and cell lines,and then analyzed the correlation between the expression of miR-449a and elinicopathologieal characteristics of colorectal cancer.We preliminarily explore the role of miR-449a in carcinogenesis and development of colorectal cancer.Methods The experimental group detected the relative expression of miR-449a in24cases of colorectal cancer tissues and in4colorectal cancer cells compared to the matched non-tumor adjacent tissues and normal colon cell by real-time RT-PCR;ELISA was used to detect the CEA expression level of24cases of blood samples.Then paired t-test was used to analyze the association between miR-449a expression and clinicopathological characteristics in colorectal cancerResults MiR-449a is upregulated in colorectal cancer tissues and cells,compared to adjacent non-tumor tissues and normal colon cells.MiR-449a negatively correlates with the serum CEA level,but have not significant correlation with patient’s age, sex, tumor location, tumor differentiation and TNM stage.Conclusions MiR-449a is upregulated in colorectal cancer tissues and cells.The expression of miR-449a negatively correlates with the serum CEA level,but have not significant correlation with patient’s age, sex, tumor location, tumor differentiation and TNM stage.5figures,3tables,27references.