The Study On The Modified Hydroxy Apatite Nanoparticles’ Capacity And Its Gene Transfection In Vitro

Objective Test the modified hydroxyapatite nanoparticles (nHAP)’s physicochemical parameters. And observe its transfection efficiency in vitro.Methods Hydroxyapatite nanoparticles (nHAP) were prepared by a chemical coprecipitation-hydrothermal synthesis. Surface modified with PEI to improve hydroxyapatite nanoparticles carrier DNA binding ability and transfection efficiency.And use the cell penetrating peptides(CPPs) to further improve the transfection efficiency, the modified nHAP/PEI-nHAP were determined for their capacity of suspension stability, DNA binding and protection, transfection efficiency of vitro cell.Results1) Hydroxyapatite nanoparticles (nHAP) were prepared by a chemical coprecipitation-hydrothermal synthesis.The nHAP have good capacity of suspension stability, DNA binding (the mass ratio is50:1)and protection.and Uniform shape and size.2) the modified nHAP after surface modification with PEI had better suspension stability, DNA binding(the mass ratio is12.5:1) and transfection efficiency is improved from1.83%to8.14%. And with the use of the cell penetrating peptides(CPPs) the transfection efficiency was improved to10.12%.Conclusion1)The modified nHAP after surface modification with PEI had better suspension stability, DNA binding and transfection efficiency.2)The cell penetrating peptides can be used to improve the transfection efficiency.(28figures,3tables,52references)...